Figures & data
Table 1. Primer sequences of qRT-PCR
Figure 1. qRT-PCR detection of miR-212-3p expression in placenta tissues of HC group and FGR group
Figure 2. Promotion of the invasion, migration and proliferation of HTR-8/SVneo and apoptosis inhibition by down-regulation expression of miR-212-3p
(a) Transfection efficiency of miR-212-3p in HTR-8/SVneo detected by qRT-PCR; (b) Cell proliferation detected by MTT assay; (c) Apoptosis detected by flow cytometry; (d) Cell invasion and migration detected by Transwell assay. **P < 0.01 and ***P < 0.001.
Figure 3. Activation of the signaling pathway of PI3K/AKT by down-regulation expression of miR-212-3p. ***P < 0.001
Figure 4. miR-212-3p can target and regulate PGF
(a) Expression levels of PGF and mRNA in the FGR and HC groups detected by qRT-PCR, *** P < 0.001; (b) PGF expression in the HC and FGR groups detected by Western blot, ***P < 0.001; (c) PGF expression detected by qRT-PCR, ***P < 0.001; (d) PGF expression detected by Western blot, ***P < 0.001; € Negative correlation of miR-212-3p with PGF on the mRNA expression; (f) PGF mRNA and miR-141-3p sequence were schematically depicted; (g) Verification of the target connection between PGF and miR-212-3p detected by Dual-luciferase reporter assay, **P < 0.01.
Figure 5. Reversion of the functional effects of PGF down-regulation on HTR-8/SVneo by down-regulation expression of miR-212-3p
(a) Cell proliferation detected by MTT assay; (b) Apoptosis detected by flow cytometry and quantitative analysis; (c) Cell invasion and migration detected by Transwell assay, ** P < 0.01, ***P < 0.001; ##P < 0.01 and ###P < 0.001.
