Figures & data
Figure 1. High levels of Salusin-β and miR-155-5p in the kidney tissues of DN mice and HG-induced renal tubular epithelial cells (HK-2 cells). (a) Levels of BUN and Scr in serum samples. (b) Level of ACR in urine samples. (c) RT-qPCR analysis of Salusin-β mRNA level in the kidney tissues of mice. (d) Western blotting analysis of Salusin-β protein level in the kidney tissues of mice. (e) RT-qPCR analysis of miR-155-5p level in the kidney tissues of mice. (f) RT-qPCR analysis of Salusin-β mRNA level in HK-2 cells. (g) Western blotting analysis of Salusin-β protein level in HK-2 cells. (h) RT-qPCR analysis of miR-155-5p level in HK-2 cells
Figure 2. Downregulation of Salusin-β reduced miR-155-5p expression. (a) RT-qPCR analysis of Salusin-β mRNA level in HG-induced HK-2 cells that were transfected with sh-Salusin-β-1 or sh-Salusin-β-2. (b) Western blotting analysis of Salusin-β protein level in HG-induced HK-2 cells that were transfected with sh-Salusin-β-1 or sh-Salusin-β-2. (c) RT-qPCR analysis of miR-155-5p level in HG-induced HK-2 cells that were transfected with sh-Salusin-β-1. (d) RT-qPCR analysis of miR-155-5p level following transfection with miR-155-5p mimic
Figure 3. Downregulation of Salusin-β alleviated HG-induced inflammatory injury and oxidative stress by suppressing miR-155-5p. (a) The release of inflammatory cytokines including TNF-α, IL-1β and IL-6 were measured using ELISA Kits. (b) Measurement of ROS, SOD and CAT activities
Figure 4. Downregulation of Salusin-β repressed the apoptosis of HG-induced HK-2 cells by suppressing miR-155-5p. Flow-cytometric analysis for detection of the apoptotic rate of HK-2 cells
Figure 5. Downregulation of Salusin-β repressed the apoptosis of HG-induced HK-2 cells by suppressing miR-155-5p. (a) TUNEL staining for the assessment of cell apoptosis. (b) Quantitative analysis of cell apoptosis
Figure 6. Downregulation of Salusin-β impeded lipid accumulation in HG-induced HK-2 cells by suppressing miR-155-5p. (a) Oil red O staining of the intracellular lipid droplet. (b) Relative TG concentrations
