Effects of two inhibitors of metabolic glutamate receptor 5 on expression of endogenous homer scaffold protein 1 in the auditory cortex of mice with tinnitus

Figures & data

Figure 1. The daily tinnitus scores, weight of body and midbrain of mice in the control group and the salicylate group. (a) Tinnitus scores of mice after saline or salicylate injection for 4 days. (b) Body weight of mice in the control group and the salicylate group after injections of saline or salicylate for 4 days. (c) Weight of midbrain of mice after injections of saline or salicylate for 4 days. ***p < 0.001

Figure 2. Expression of Homer1/2/3 in the auditory cortex of mice. (a) Homer1 mRNA and protein is expressed in the auditory cortex (temporal cortex area 3) of mice in the salicylate group. Scale bar: 500 μm. Red arrow points to Homer1 protein (bluish violet) and black arrow points to Homer1 mRNA (claybank). (b) Relative Homer1 mRNA expression in the auditory cortex of mice after injections of saline or salicylate on Day 1, Day 2, Day 3, Day 4 was detected by PCR analysis. (c) Relative Homer1 protein expression in the auditory cortex of mice after injections of saline or salicylate on Day 1, Day 2, Day 3, Day 4 was detected by western blotting analysis. (d-e) Relative Homer2 expression at the mRNA and protein levels in the auditory cortex of mice in the control group and the salicylate group on Day 1, Day 2, Day 3, Day 4 was detected by PCR and western blotting analysis. (f-g) Relative Homer3 expression at the mRNA and protein levels in the auditory cortex of mice in the control group and the salicylate group on Day 1, Day 2, Day 3, Day 4 was detected by PCR and western blotting analysis. *p < 0.05, **p < 0.01

Figure 3. Effects of MPEP and MCPG on the tinnitus scores, weight of body and midbrain of mice in the salicylate group. (a) Daily tinnitus scores of mice injected with salicylate combined with different concentrations of MPEP (0, 100, 250, 500 nmol/L). (b) Daily tinnitus scores of mice injected with salicylate combined with different concentrations of MCPG (0, 100, 250, 500 nmol/L). (c) Body weight of mice injected with salicylate combined with different concentrations of MPEP (0, 100, 250, 500 nmol/L) was evaluated on the 4^th day after injections. (d) Body weight of mice injected with salicylate combined with different concentrations of MCPG (0, 100, 250, 500 nmol/L) was evaluated on the 4^th day after injections. *p < 0.05, **p < 0.01

Figure 4. Homer1 expression was inhibited by MPEP but not MCPG in the auditory cortex of mice in the salicylate group. (a) Relative mRNA expression of HOMER1 in the auditory cortex of mice injected with salicylate combined with different concentrations of MPEP (0, 100, 250, 500 nmol/L). (b) Relative mRNA expression of HOMER1 in the auditory cortex of mice injected with salicylate combined with different concentrations of MCPG (0, 100, 250, 500 nmol/L). (c) Relative protein expression of HOMER1 in the auditory cortex of mice injected with salicylate combined with different concentrations of MPEP (0, 100, 250, 500 nmol/L). (d) Relative protein expression of HOMER1 in the auditory cortex of mice injected with salicylate combined with different concentrations of MCPG (0, 100, 250, 500 nmol/L). (e-f) Relative mRNA and protein expression of HOMER1 in the auditory cortex of mice injected with saline combined with different concentrations of MPEP (0, 100, 250, 500 nmol/L) were detected by PCR and western blotting analysis. *p < 0.05, **p < 0.01