Figures & data
Figure 1. The expression of TRIM22 was promoted in cataract tissues. (a, b) The expression of TRIM22 in cataract tissues was detected with the RT-PCR and western blotting. (c) Immunohistochemical staining was performed for the detection of the expression of TRIM22 in cataract tissues. (d, e) The expression of TRIM22 in lens epithelial cells was detected with the RT-PCR and western blotting. ** p < 0.01 (Control vs H2O2)
Figure 2. Knockdown of TRIM22 suppressed the proliferation of lens epithelial cells. (a, b) The expression of TRIM22 in lens epithelial cells was determined with the RT-PCR and western blotting. (c) CCK-8 was performed for the detection of the proliferation of lens epithelial cells. (d) EdU assays were performed for the detection of the proliferation of lens epithelial cells. ** p < 0.01, ## p < 0.05 (H2O2+ shNC vs H2O2+ TRIM22)
Figure 3. Knockdown of TRIM22 relieved the apoptosis of lens epithelial cells. (a) The apoptosis of lens epithelial cells was detected with the TUNEL staining. (b) The expression of apoptosis related proteins in lens epithelial cells was determined with the western blotting. (c, d) The expression of γ-H2AX in these cells was determined with the immunofluorescence and western blotting. ** p < 0.01, ## p < 0.05
Figure 4. Knockdown of TRIM22 relieved the oxidative stress of lens epithelial cells. (a) The levels of LDH in lens epithelial cells was determined with the commercial kits. (b) The production of ROS was detected with the commercial kits. (c) The levels of MDA, SOD and GSH-PX were determined with the commercial kits. ** p < 0.01, ## p < 0.05
Figure 5. Inhibition of TRIM22 suppressed the expression of TRAF6. (a) The expression of TRAF6 in these cells were determined with the western blotting. (b) The expression of p-p38 and p-ERK in lens epithelial cells was determined with the western blotting. (c) The expression of TRAF6, p-p38 and p-ERK in lens epithelial cells was detected with the western blotting. ** p < 0.01, ## p < 0.05
