1,202
Views
3
CrossRef citations to date
0
Altmetric
Research Paper

Preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability

, , , , , , & show all
Pages 8031-8042 | Received 14 Jun 2021, Accepted 12 Sep 2021, Published online: 20 Oct 2021

Figures & data

Figure 1. Schematic illustration of electro-spinning process

Figure 1. Schematic illustration of electro-spinning process

Figure 2. Protein production. (a) Schematic structure of SUMO-GMCDRSSP-IGF-1 spidroin. (b) The identification of the protein production. Lanes 1–9 are protein ladder, total protein before induction, total protein after induction, supernatant of cell lysate, precipitation of cell lysate, elution solution, mixture after ULP1 protease cleavage, GMCDRSSP-IGF-1 spidroin, and SUMO protein, respectively

Figure 2. Protein production. (a) Schematic structure of SUMO-GMCDRSSP-IGF-1 spidroin. (b) The identification of the protein production. Lanes 1–9 are protein ladder, total protein before induction, total protein after induction, supernatant of cell lysate, precipitation of cell lysate, elution solution, mixture after ULP1 protease cleavage, GMCDRSSP-IGF-1 spidroin, and SUMO protein, respectively

Figure 3. SEM images of nanofibrous membrane and diameter distribution histogram

Figure 3. SEM images of nanofibrous membrane and diameter distribution histogram

Figure 4. Characteristics of the nanofibrous membrane. (a) CD spectra. (b) WAXD pattern. (c) FITR spectra. (d) Typical stress-strain curve. (e) TG and DTG curves. (f) Water contact angle (up) and hemolysis assays (bottom). (+), (-) and (m) represent positive controls, negative controls, and nanofibrous membrane

Figure 4. Characteristics of the nanofibrous membrane. (a) CD spectra. (b) WAXD pattern. (c) FITR spectra. (d) Typical stress-strain curve. (e) TG and DTG curves. (f) Water contact angle (up) and hemolysis assays (bottom). (+), (-) and (m) represent positive controls, negative controls, and nanofibrous membrane

Figure 5. Degradation testing results of GMCDRSSP-IGF-1 spidroins in vitro. SEM images of degraded nanofibrous membrane (Left) and degradation curve (Right)

Figure 5. Degradation testing results of GMCDRSSP-IGF-1 spidroins in vitro. SEM images of degraded nanofibrous membrane (Left) and degradation curve (Right)

Figure 6. Controlled sustained-release performance test in vitro of GMCDRSSP-IGF1 spidroins

Figure 6. Controlled sustained-release performance test in vitro of GMCDRSSP-IGF1 spidroins

Figure 7. Effects on the proliferation of EPCs cultured on GMCDRSSP-IGF-1 nanofibrous membrane in vitro.

Figure 7. Effects on the proliferation of EPCs cultured on GMCDRSSP-IGF-1 nanofibrous membrane in vitro.