Figures & data
Figure 1. Tranilast inhibited AngII-induced overproliferation and migration of HCF cells. A. CCK-8 assay detected the cell viability after induction with different concentrations of Tranilast. B. CCK-8 assay detected the cell viability of AngII-induced cells after induction with different concentrations of Tranilast. C and D. wound healing detected the migration of cells. E. Western blot detected the expression of MMP2 and MMP9. *P < 0.05, **P < 0.01, ***P < 0.001 vs control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs Ang II
Figure 2. Tranilast inhibited AngII-induced fibrosis of HCF cells. A. The expression of proteins related to ECM synthesis was detected by Western blot. B. The expression of α-SMA was detected by IF. ***P < 0.001 vs control; ##P < 0.01, ###P < 0.001 vs Ang II
Figure 3. Tranilast down-regulated the expression of S100A11. A. RT-qPCR detected the expression of S100A11. B. Western blot detected the expression of S100A11. ***P < 0.001 vs control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs Ang II
Figure 4. Overexpression of S100A11 reversed the inhibitory effect of Tranilast on AngII-induced overproliferation and migration of HCF cells. A. RT-qPCR detected the expression of S100A11 after cell transfection. B. Western blot detected the expression of S100A11 after cell transfection. ***P < 0.001 vs Oe-NC. C. CCK-8 assay detected the cell viability of AngII-induced cells after induction with different concentrations of Tranilast and cell transfection. D. Western blot detected the expression of MMP2 and MMP9. E. wound healing detected the migration of cells. ***P < 0.001 vs control; ###P < 0.001 vs Ang II; ΔP<0.05, ΔΔP<0.01, ΔΔΔP<0.001 vs Ang II + Tranilast + Oe-NC
Figure 5. Overexpression of S100A11 reversed the inhibitory effect of Tranilast on AngII-induced fibrosis of HCF cells. A. The expression of proteins related to ECM synthesis was detected by Western blot. B. The expression of α-SMA was detected by IF. ***P < 0.001 vs control; ###P < 0.001 vs Ang II; ΔP<0.05, ΔΔP<0.01, ΔΔΔP<0.001 vs Ang II + Tranilast + Oe-NC
Figure 6. Tranilast regulated the TGF-β1/Smad pathway. The expression of proteins related to TGF-β1/Smad pathway was detected by Western blot. ***P < 0.001 vs control; ###P < 0.001 vs Ang II; ΔΔP<0.01, ΔΔΔP<0.001 vs Ang II + Tranilast + Oe-NC
