LncRNA ROR/miR-145-5p axis modulates the osteoblasts proliferation and apoptosis in osteoporosis

Figures & data

Table 1. Clinical data of the study population

Factors	Control (n = 79)	OP patients (n = 82)	P value
Age (years)	49.68 ± 4.17	50.48 ± 3.5	0.193
Gender (male/female)	21/58	22/60	0.972
BMI (kg/m^2)	23.88 ± 3.04	24.05 ± 3.04	0.176
Lumbar spine bone mineral density (g/cm^2)	0.90 ± 0.05	0.80 ± 0.03	<0.001
Femoral neck bone mineral density (g/cm^2)	0.71 ± 0.04	0.64 ± 0.04	<0.001
Total hip bone mineral density (g/cm^2)	0.75 ± 0.04	0.67 ± 0.03	<0.001

OP: osteoporosis; BMI, body mass index; data are expressed as n or mean ± standard deviation. Differences between groups were compared using Student’s t test for continuous variables, and chi-squared test for categorical variables.

Table 2. Correlation between lncRNA ROR and clinical characteristics

Characteristics	Correlation with lncRNA ROR (r)	P-value
Age (years)	0.121	0.278
Gender (male/female)	0.097	0.388
BMI (kg/m^2)	0.075	0.505
Lumbar spine bone mineral density (g/cm^2)	−0.369	0.001
Femoral neck bone mineral density (g/cm^2)	−0.351	0.001
Total hip bone mineral density (g/cm^2)	−0.465	<0.001

BMI, body mass index. The correlation of different indicators was calculated through Pearson’s correlation analysis.

Figure 1. (a) lncRNA ROR levels in the serum of OP patients and healthy controls using qRT-PCR. (b) Levels of miR-145-5p in the serum of OP patients in comparison with the control group. *** P < 0.001. Differences between groups were compared using student’s t test

Figure 2. The diagnostic value of serum ROR (a) and miR-145-5p (b) for OP. The AUC of the combined diagnosis of ROR and miR-145-5p was 0.925 (c), which is higher than the diagnostic value of a single indicator

Figure 3. (a) The levels of ROR in different MC3T3-E1 cell groups. (b) The cell viability assessment based on CCK-8 assay. (c) Cell apoptosis evaluation using flow cytometry assay. *** P < 0.001, compared with control group. Differences among groups were compared using one-way ANOVA

Figure 4. (a) STARBASE predicted the binding sites between lncRNA ROR and miR-145-5p. (b) The luciferase activity of cells transfected with WT-ROR vector or MUT ROR vector and miR-145-5p mimic, or inhibitor. *** P < 0.001, compared with control group. (c) Levels of miR-145-5p in different MC3T3-E1 cell groups. *** P < 0.001, compared with control group. (d) Association between serum ROR and miR-145-5p levels. Differences among groups were compared using one-way ANOVA

Figure 5. (a) qRT-PCR results for the measurement of miR-145-5p levels in different cell groups. (b) CCK-8 assay results reflecting the cell proliferation in different groups. (c) Cell apoptosis evaluation using flow cytometry assay. *** P < 0.001, compared with control group; ^&&& P < 0.001, compared with si-ROR group. Differences among groups were compared using one-way ANOVA

Figure 6. (a) Bioinformatics analysis showed that miR-145-5p contains binding sites for ROCK1. (b) The luciferase activity of different cell groups. *** P < 0.001, compared with control group

Data availability statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.