LRG1 promotes hypoxia-induced cardiomyocyte apoptosis and autophagy by regulating hypoxia-inducible factor-1α

Figures & data

Table 1. LASSO analysis results of 14 common DEGs in 3 datasets

	GSE62646	GSE66360	GSE123342
(Intercept)	−68.6401	−17.5185	−0.9315
BCL6	0	1.009885	−1.26556
SLC11A1	0	1.277634	2.567696
LILRB2	0.153351	0.036732	1.77565
CDA	0	0.517451	0
ZFP36	2.422645	1.170204	0.995548
AQP9	0	0	0.768797
LRG1	0.280353	0.239079	0.752645
CSF3R	0.080199	0	−5.21579
DYSF	0	0.17646	0
LILRA2	0	−0.30752	0
CYP1B1	0.360468	0.449016	0.864412
CFP	3.222292	0	0
GZMK	0	−0.99028	−0.68488
STEAP4	0	−1.05143	1.196072

The numbers in the table represent the coefficients of each gene in the LASSO model.

Figure 1. Bioinformatics analysis results. (a) The differential genes heatmap of GSE62646, GSE66360 and GSE123342; (b) Venn diagram of DEGs; (c) GO enrichment analysis of 14 common DEGs; (d) KEGG enrichment analysis of 14 common DEGs; (e) PPI analysis of common differential genes; (f) The expression of four key genes at different stages in GSE123342

Figure 2. Effect of hypoxia on H9c2. (a) Cell viability was detected by CCK-8 after hypoxia treatment (0, 4, 8, 12, 18, 24 h); (b) Apoptosis was detected by Annexin V-FITC/PI staining; (c) Autophagy was detected by immunofluorescence assay (the scale bars = 50 μm); (d) Western blot was used to detect the expression of Bax, Bcl2, LC3, p62 and LRG1 protein. *P < 0.05, **P < 0.01, ***P < 0.001

Figure 3. Effect of LRG1 on cell viability and cell apoptosis in H9c2. (a) The interference effect of siLRG1 was detected by western blot; (b) Cell viability was detected by CCK-8; (c) Apoptosis was detected by Annexin V-FITC/PI staining; (d) Western blot was used to detect the expression of Bax and Bcl2 protein. When normoxia as control, ***P < 0.001. When hypoxia + siNC as control, ^##P < 0.01, ^###P < 0.001

Figure 4. Effect of LRG1 on autophagy. (a) Autophagy was detected by immunofluorescence assay (the scale bars = 50 μm); (b) LC3 and p62 protein expression were detected by western blot

Figure 5. Effect of LRG1 on HIF-1α in normoxia H9c2. (a) Western blot was applied to detect the effect of LRG1 overexpression plasmid and siLRG1; (b) Western blot was used to detect HIF-1α protein expression

Figure 6. siHIF-1α reversed the effect of LRG1 on H9c2 viability and cell apoptosis. (a) The interference effect of siHIF-1α was detected by western blot; (b) Cell viability was detected by CCK-8; (c) Apoptosis was detected by Annexin V-FITC/PI staining; (d) Western blot was used to detect the expression of Bax and Bcl2 protein. When normoxia as control, ***P < 0.001. When hypoxia+OE-NC as control, ^###P < 0.001. When hypoxia+LRG1+ siNC as control, ^&&P < 0.01

Figure 7. siHIF-1α reversed the effect of LRG1 on autophagy in H9c2. (a) Autophagy was detected by immunofluorescence assay (the scale bars = 50 μm); (b) LC3 and p62 protein expression were detected by western blot

Data availability statement

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.