Figures & data
Table 1. Primer sequences
Figure 1. The effect of up-regulating the expression of lncRNA DANCR on the trophoblast cell cycle and cell proliferation
(a) qRT-PCR for evaluating the efficiency of overexpressing (pc-NC, DANCR) and interfering with the lncRNA DANCR (sh-NC, sh-DANCR); (b) determination of the cell proliferation of each group of cells using MTT; (c) measurement of cell cycles in each group using flow cytometry; #p < 0.05, ##p < 0.01 and ###p < 0.001 vs ., pc-NC group. *p < 0.05, **p < 0.01 and ***p < 0.001 vs., sh-NC group.
Figure 2. Up-regulation of lncRNA DANCR can promote the migration, invasion, and epithelial-mesenchymal transition of trophoblast cells
(a) Assessment of the invasion ability using Transwell; (b) scratch test for observing the migration ability; (c) Western blot for determining the E-cadherin, vimentin, N-cadherin, and Twist expression; #p < 0.05 and ##p < 0.01 vs., pc-NC group. *p < 0.05 and **p < 0.01 vs., sh-NC group.
Figure 3. Connection of miR-214-5p and lncRNA DANCR
(a) Verification of the targeted interaction of miR-214-5p and lncRNA DANCR by dual-luciferase assay, **p < 0.01 vs., NC group; (b) qRT-PCR for evaluating the miR-214-5p expression in trophoblast cells, #p < 0.05 vs., pc-NC group, **p < 0.01 vs., sh-NC group.
Figure 4. lncRNA DANCR regulates the preeclampsia progress by binding to miR-214-5p
(a) MTT for determining the proliferation of HTR-8/SVneo cells; (b) flow cytometry for assessing the cell cycle of HTR-8/SVneo cells; (c) measurement of the invasion ability of HTR-8/SVneo cells by Transwell experiment; (d) scratch test for evaluating the migration ability of HTR-8/SVneo cells; (e) Western blot for assessing the EMT-related protein expression; *p < 0.05 and **p < 0.01 vs., pc-NC group, #p < 0.05 and ## p < 0.01 vs., DANCR group.
Figure 5. The function of lncRNA DANCR on proteins related to PI3K/AKT pathway
&&p < 0.01 vs., DANCR+LY294002 group, ##p < 0.01 vs DANCR group, **p < 0.01 vs., pc-NC group.
