Figures & data
Figure 1. Proliferation is suppressed and apoptosis is induced by AZD3759 in glioma cells. a. The clonogenic assay used to determine the proliferation ability of cells. b. The apoptotic rate of treated glioma cells detected by flow cytometry. The Q2 + Q3 flow cytometry gates are used for the calculation of apoptotic rate. c. The cell cycle of treated glioma cells detected by flow cytometry (*p < 0.05 vs. control, **p < 0.01 vs. control, #p < 0.05 vs. 4 μM AZD3759)
Figure 2. The EGFR and JAK/STAT pathways in glioma cells are inhibited by AZD3759. a. The expression levels of JAK1, p-JAK1, JAK2, p-JAK2, STAT3, p-STAT3, STAT5, p-STAT5, EGFR, p-EGFR, AKT, and p-AKT in C6 cells detected using western blot assay. b. The expression levels of JAK1, p-JAK1, JAK2, p-JAK2, STAT3, p-STAT3, STAT5, p-STAT5, EGFR, p-EGFR, AKT, and p-AKT in U87 cells detected using western blot assay (*p < 0.05 vs. control, **p < 0.01 vs. control, ##p < 0.01 vs. 4 μM AZD3759)
Figure 3. Luciferase activity is positively related to the number of C6-LUC cells. a. The luciferin activity in C6-WT and C6-LUC cells determined using the IVIS spectrum system (**p < 0.01 vs. C6-WT). b. The photons of the different number of C6-LUC cells detected by the IVIS spectrum system. c. The OD value of C6-WT and C6-LUC cells measured using CCK-8 assay. d. The cell cycle of C6-WT and C6-LUC cells determined using flow cytometry assay
Figure 4. AZD3759 suppresses the growth of C6 cells in xenograft mice. a. Live images of animals of each group taken 1 week and 3 weeks after treatment. b. Average signals quantified using the IVIS spectrum system (*p < 0.05 vs. control, **p < 0.01 vs. control, #p < 0.05 vs. 60 mg/kg AZD3759). c. Tumor volumes calculated at the end of the experiments (*p < 0.05 vs. control, **p < 0.01 vs. control, ##p < 0.01 vs. 60 mg/kg AZD3759)
Figure 5. AZD3759 suppresses the EGFR and JAK/STAT signaling pathways in xenograft C6 tissues. The expression levels of JAK1, p-JAK1, JAK2, p-JAK2, STAT3, p-STAT3, STAT5, p-STAT5, EGFR, p-EGFR, AKT, and p-AKT in tumor tissues detected using western blot assay (*p < 0.05 vs. control, **p < 0.01 vs. control, ##p < 0.01 vs. 60 mg/kg AZD3759)
