Figures & data
Figure 1. Expression of miR-144 and DNAJC3-AS1 in MCF-7 cells and MCF-7/DOX cells. (a) The relative cell viability was detected by MTT assay. (b) mRNA expression of miR-144 and DNAJC3-AS1 mRNA expression was detected. (c) The expression of DNAJC3-AS1, Atg5, LC3-II and LC3-I protein expression was detected. **P < 0.01vs. MCF-7 group
![Figure 1. Expression of miR-144 and DNAJC3-AS1 in MCF-7 cells and MCF-7/DOX cells. (a) The relative cell viability was detected by MTT assay. (b) mRNA expression of miR-144 and DNAJC3-AS1 mRNA expression was detected. (c) The expression of DNAJC3-AS1, Atg5, LC3-II and LC3-I protein expression was detected. **P < 0.01vs. MCF-7 group](/cms/asset/382edf78-394d-4a58-937d-62bf04dccd13/kbie_a_1999373_f0001_oc.jpg)
Figure 2. miR-144 overexpression improves the sensitivity and induces apoptosis of drug-resistant MCF-7 cells. (a) The expression of Mir-144 mRNA was detected. (b) Cell viability was detected by MTT. (c) The apoptosis of cells was evaluated by flow cytometry. **P < 0.01 vs. Control group
![Figure 2. miR-144 overexpression improves the sensitivity and induces apoptosis of drug-resistant MCF-7 cells. (a) The expression of Mir-144 mRNA was detected. (b) Cell viability was detected by MTT. (c) The apoptosis of cells was evaluated by flow cytometry. **P < 0.01 vs. Control group](/cms/asset/addbc53e-2abc-4fe7-a66e-e462c02de147/kbie_a_1999373_f0002_oc.jpg)
Figure 3. miR-144 targeted regulation of DNAJC3-AS1 expression to detect the sensitivity of drug-resistant MCF-7 cells. (a) Using TargetScan predict the binding sites of miR-144 and DNAJC3-AS1. (b) The targeted relationship between miR-144 and DNAJC3-AS1 was analyzed by Luciferase activity. (c) The DNAJC3-AS1 protein expression was detected. (d) Determination of cell viability. (e) Cell apoptosis was detected. *P < 0.05, **P < 0.01 vs. Control group, ##P < 0.01 vs. NC inhibitor
![Figure 3. miR-144 targeted regulation of DNAJC3-AS1 expression to detect the sensitivity of drug-resistant MCF-7 cells. (a) Using TargetScan predict the binding sites of miR-144 and DNAJC3-AS1. (b) The targeted relationship between miR-144 and DNAJC3-AS1 was analyzed by Luciferase activity. (c) The DNAJC3-AS1 protein expression was detected. (d) Determination of cell viability. (e) Cell apoptosis was detected. *P < 0.05, **P < 0.01 vs. Control group, ##P < 0.01 vs. NC inhibitor](/cms/asset/0409933d-b25b-4ed8-9ff9-d408be815f83/kbie_a_1999373_f0003_oc.jpg)
Figure 4. miR-144 increases the chemosensitivity of breast cancer cells by inhibiting the expression of autophagy-related proteins and targeting DNAJC3-AS1. (a) Detection of cell activity. (b) Cell apoptosis was detected. (c) Autophagy related protein expression was detected. **P < 0.01 vs. miR-control group, #P < 0.05, ##P < 0.01 vs. miR-144+ pcDNA 3.0 group
![Figure 4. miR-144 increases the chemosensitivity of breast cancer cells by inhibiting the expression of autophagy-related proteins and targeting DNAJC3-AS1. (a) Detection of cell activity. (b) Cell apoptosis was detected. (c) Autophagy related protein expression was detected. **P < 0.01 vs. miR-control group, #P < 0.05, ##P < 0.01 vs. miR-144+ pcDNA 3.0 group](/cms/asset/06049b0d-c7a4-4347-b224-59b901f8f549/kbie_a_1999373_f0004_oc.jpg)