Clinical value of lncRNA TUG1 in temporal lobe epilepsy and its role in the proliferation of hippocampus neuron via sponging miR-199a-3p

Figures & data

Table 1. Baseline characteristics of the subjects

Characteristics	Healthy individuals (n = 86)	TLE children (n = 88)	P value
Mean (SD) age,years	10.06 (3.07)	9.72 (3.27)	0.506
Gender (male/female), n	48/38	53/35	0.555

Note: TLE, temporal lobe epilepsy; SD, standard deviation.

Figure 1. Serum expression levels of TUG1 in children with temporal lobe epilepsy (TLE) and healthy controls. The expression of TUG1 in TLE children was significantly increased. *** P < 0.001, compared with the healthy individuals

Figure 2. A receiver operating characteristic ROC curve was conducted to calculate the diagnostic ability of serum TUG1 for TLE. The area under the curve (AUC) for TUG1 was 0.915, with a sensitivity of 80.7% and specificity of 88.4% at the cutoff value of 1.256

Figure 3. Effect of TUG1 on cell viability and apoptosis of hippocampal neurons. (a) primary hippocampal cells of newborn rats were cultured in the magnesium-free medium for three hours to simulate the symptoms of TLE in children. High expression of TUG1 was observed in cells treated in the magnesium-free medium compared with the untreated cells. Transfection of si-TUG1 led to a remarkable decrease in the expression level of TUG1. (b) No Mg²⁺ treatment reduced the cell viability, but the cell viability was promoted by knocking out TUG1. (c) No Mg²⁺ treatment promoted cell apoptosis, however, knocking out TUG1 inhibited TLE – induced apoptosis. *** P < 0.001, compared with untreated group; ^### P < 0.001, compared with no Mg²⁺ group

Figure 4. MiR-199a-3p was a direct target of TUG1. (a) the binding site of TUG1 in miR-199a-3p. (b) miR-199a-3p mimic significantly inhibited the luciferase activity of WT 3 ‘-UTR of TUG1, while the luciferase activity of miR-199a-3p inhibitor was the opposite. Furthermore, the transfection of miR-199a-3p mimic or miR-199a-3p inhibitor did not affect luciferase activity in the mutant group. (c) compared with the control group, the expression level of miR-199a-3p was significantly decreased in the no Mg²⁺ group and significantly increased in the si-TUG1group. (d) the expression level of miR-199a-3p was significantly reduced in children with TLE compared with healthy individuals. (e) the expression of TUG1 in TLE children was negatively correlated with miR-199a-3p level (r = −0.7223, P < 0.0001). *** P < 0.001, compared with the untreated group, control group, or healthy individuals. ^### P < 0.001, compared with No Mg²⁺ group