Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion, and migration of breast cancer cells by targeting miR-331

Figures & data

Figure 1. LncRNA ANCR expressions in breast cancer tissues (n = 48) and cells (n = 3). **Compared with MCF10A cells, p < 0.001; #compared with paracancerous tissue, p < 0.05

Figure 2. LncRNA ANCR expressions in cells after transfection. All experiments were performed in triplicate independently. *Compared with MDA-MB-231 (MCF-7)-shANCR cells, p < 0.05

Table 1. Correlations between lncRNA ANCR expression and clinicopathological characteristics of patients with breast cancer

Characteristic	High expression (n = 15) (%)	Low expression (n = 33) (%)	χ^2	p
Age (year)			0.257	0.613
≤40	7	18
>40	8	15
Tumor volume (cm^3)			8.394	0.004
≤2	9	6
>2	6	27
Clinical stage			Fisher’s	0.025
I	6	5
II	5	4
III	4	21
IV	0	3
Lymph node metastasis			5.610	0.018
Yes	5	23
No	10	10
Subtype			8.097	0.044
Luminal A	2	8
Luminal B	8	5
HER-2 overexpression	2	12
Basal-like	3	8

Figure 3. Effect of lncRNA ANCR on MDA-MB-231 (MCF-7) cell proliferation. All experiments were performed in triplicate independently. A: MTT assay results; B: colony formation assay results

Figure 4. Effect of lncRNA ANCR on MDA-MB-231 (MCF-7) cell invasion. All experiments were performed in triplicate independently. Compared with MDA-MB-231-shANCR cells, *p < 0.05

Figure 5. Effect of lncRNA ANCR on MDA-MB-231 (MCF-7) cell migration. All experiments were performed in triplicate independently. Compared with MDA-MB-231-shANCR cells, *p < 0.05

Figure 6. Effect of lncRNA ANCR on tumor formation in nude mice (n = 8). A: Tumor volumes; B: tumor weights. Compared with MDA-MB-231-shANCR group, *p < 0.05

Figure 7. Relationship between lncRNA ANCR and miR-331. A: Dual luciferase reporter assay results; B: RT-PCR results. Compared with MDA-MB-231-shANCR group, *p < 0.05; C: miR-331 expressions in breast cancer and adjacent tissues (n = 48). All experiments were performed in triplicate independently