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Research Paper

Correlation between mechanism of oxidized-low density lipoprotein-induced macrophage apoptosis and inhibition of target gene platelet derived growth factor receptor-β expression by microRNA-9

, , , , &
Pages 11716-11725 | Received 16 Aug 2021, Accepted 10 Nov 2021, Published online: 11 Dec 2021

Figures & data

Figure 1. Diagram of the test process (arrows with different colors and different line types referred to different test processes)

Figure 1. Diagram of the test process (arrows with different colors and different line types referred to different test processes)

Figure 2. Observation of macrophage RAW264.7 oil red O staining (×100)

Figure 2. Observation of macrophage RAW264.7 oil red O staining (×100)

Figure 3. Macrophage RAW264.7 proliferation detection (* indicated that the difference between groups was statistically significant, P < 0.05)

Figure 3. Macrophage RAW264.7 proliferation detection (* indicated that the difference between groups was statistically significant, P < 0.05)

Figure 4. Macrophage RAW264.7 apoptosis detection (* indicated that the difference between groups was statistically significant, P < 0.05)

Figure 4. Macrophage RAW264.7 apoptosis detection (* indicated that the difference between groups was statistically significant, P < 0.05)

Figure 5. Detection of Caspase-3 and GRP78 protein expression levels in macrophages RAW264.7 (* indicated that the difference between groups was statistically significant, P < 0.05)

Figure 5. Detection of Caspase-3 and GRP78 protein expression levels in macrophages RAW264.7 (* indicated that the difference between groups was statistically significant, P < 0.05)

Figure 6. Detection of PDGFR-β mRNA and protein expression levels in macrophages RAW264.7 (* indicated that the difference between groups was statistically significant, P < 0.05)

Figure 6. Detection of PDGFR-β mRNA and protein expression levels in macrophages RAW264.7 (* indicated that the difference between groups was statistically significant, P < 0.05)

Figure 7. Target prediction results of miRNA-9 and multi-species PDGFR-β 3ʹ UTR region (red bases were the target binding sites)

Figure 7. Target prediction results of miRNA-9 and multi-species PDGFR-β 3ʹ UTR region (red bases were the target binding sites)

Figure 8. Results of dual luciferase reporter gene detection (red bases were wild-type and mutant-type binding sites; and * indicated that the difference between groups was statistically significant, P < 0.05)

Figure 8. Results of dual luciferase reporter gene detection (red bases were wild-type and mutant-type binding sites; and * indicated that the difference between groups was statistically significant, P < 0.05)