MicroRNA-381-3p signatures as a diagnostic marker in patients with sepsis and modulates sepsis-steered cardiac damage and inflammation by binding HMGB1

Figures & data

Table 1. Comparison of the baseline data between the two groups

Parameters	Control (n = 105)	Sepsis (n = 102)	P value
Age (year)	55.93 ± 14.64	56.85 ± 12.69	0.630
BMI (kg/m^2)	23.21 ± 2.94	22.94 ± 3.11	0.554
Gender (male/female)	53/52	53/49	0.831
PCT (ng/mL)	0.11 ± 0.04	7.72 ± 2.88	<0.001
WBC (×10^9/L)	6.25 ± 1.39	15.51 ± 3.56	<0.001
CRP (mg/L)	6.38 ± 1.54	85.85 ± 20.16	<0.001
Albumin (g/L)	39.68 ± 3.23	28.26 ± 5.68	<0.001
Scr (mg/dL)	0.92 ± 0.19	1.68 ± 0.22	<0.001
SOFA score	-	5.02 ± 1.34	-
APACHE II score	-	12.02 ± 5.58	-

Note: BMI, body mass index; PCT, procalcitonin; WBC, white blood cell; CRP, C-reactive protein; Scr, serum creatinine; SOFA, sequential organ failure assessment; APACHE, acute physiology and chronic health evaluation.

Figure 1. A total of 105 healthy controls and 102 sepsis patients were included in this study. (a) The expression miR-381-3p in sepsis. (b) The ROC curve showed the diagnosis importance of miR-381-3p. * **P < 0.001

Table 2. Correlation between the levels of miR-381-3p and clinical characteristics in 102 patients with sepsis

Parameters	The expression of miR-381-3p
	P value	Correlation coefficient (r)
Age	0.969	0.004
BMI	0.983	0.002
Gender	0.961	−0.005
PCT	<0.001	−0.878
WBC	<0.001	−0.810
CRP	<0.001	−0.821
Albumin	0.085	0.117
Scr	<0.001	−0.668
SOFA score	<0.001	−0.852
APACHE II score	<0.001	−0.725

Note: BMI, body mass index; PCT, procalcitonin; WBC, white blood cell; CRP, C-reactive protein; Scr, serum creatinine; SOFA, sequential organ failure assessment; APACHE, acute physiology and chronic health evaluation.

Figure 2. (a) MiR-381-3p mimics reversed the declined expression of miR-381-3p in H9c2 cells. The finding of (b) cell viability, (c) apoptosis, and (d) inflammation in vitro assay. ***P < 0.001, **P < 0.01, compared to control group; &&& P < 0.001, &&P < 0.01, compared to the LPS group

Figure 3. Each group included five rats. (a) MiR-381-3p mimics reversed the declined expression of miR-381-3p in SD rats. The finding of (b) CK-MB, (c) cTnI, (d) LVEDP, (e) LVSP, (f) +dp/dtmax, and (g)-dp/dtmax, (h) LVM, and (i) LVWT. ***P < 0.001, **P < 0.01, *P < 0.05, compared to sham group; &&& P < 0.001, && P < 0.01, compared to the CLP group

Figure 4. Each group included five rats. The alternations of (a) TNF-α, (b) IL-6, and (c) IL-1β. ***P < 0.001, compared to sham group; &&& P < 0.001, compared to the CLP group

Figure 5. (a) The complementary sites between miR-381-3p and HMGB1. (b) The establishment of luciferase results. (c) overexpression of miR-381-3p restricted the mRNA expression of HMGB1, which was facilitated by the absence of miR-381-3p. ***P < 0.001, **P < 0.01