Identification and validation of long non-coding RNA associated ceRNAs in intrauterine adhesion

Figures & data

Table 1. Top 15 terms of Gene Ontology analysis of the 915 differentially expressed genes

Term	Description	Count	p Value
GO:0006323	DNA packaging	36	1.11E-10
GO:0034401	chromatin organization involved in regulation of transcription	25	4.08305E-09
GO:0045814	negative regulation of gene expression, epigenetic	22	2.58071E-08
GO:0030198	extracellular matrix organization	44	3.47E-08
GO:0003012	muscle system process	46	1.25E-07
GO:0071824	protein-DNA complex subunit organization	34	2.5606E-07
GO:0002526	acute inflammatory response	19	8.97E-07
GO:0050863	regulation of T cell activation	39	2.17E-06
GO:0040029	regulation of gene expression, epigenetic	26	3.25783E-06
GO:0010959	regulation of metal ion transport	39	3.38777E-06
GO:0009311	oligosaccharide metabolic process	12	6.77125E-06
GO:1903039	positive regulation of leukocyte cell-cell adhesion	29	2.88932E-05
GO:0062197	cellular response to chemical stress	34	3.01087E-05
GO:0007249	I-kappaB kinase/NF-kappaB signaling	30	3.03254E-05
GO:0007080	mitotic metaphase plate congression	10	4.30341E-05

Table 2. Top 15 terms of Kyoto Encyclopedia of Genes and Genomes pathway analysis of the 915 differentially expressed genes

Term	Description	Input number	p−Value
hsa05322	Systemic lupus erythematosus	37	6.09192E-20
hsa05169	Epstein-Barr virus infection	32	1.94502E-11
hsa04621	NOD-like receptor signaling pathway	27	2.26949E-09
hsa04060	Cytokine-cytokine receptor interaction	34	9.2017E-09
hsa05200	Pathways in cancer	48	1.53534E-08
hsa05202	Transcriptional misregulation in cancer	26	1.65094E-08
hsa05167	Kaposi sarcoma-associated herpesvirus infection	25	7.4316E-08
hsa04064	NF-kappa B signaling pathway	18	9.02412E-08
hsa04668	TNF signaling pathway	19	1.18615E-07
hsa04217	Necroptosis	21	1.383E-06
hsa05161	Hepatitis B	21	1.5109E-06
hsa05323	Rheumatoid arthritis	15	1.89847E-06
hsa05166	Human T-cell leukemia virus 1 infection	24	2.92875E-06
hsa04620	Toll-like receptor signaling pathway	16	3.27057E-06
hsa05152	Tuberculosis	21	3.82689E-06

Figure 1. The protein–protein interaction network of differentially expressed genes.

Figure 2. The ceRNAs network in IUA. Diamond-shaped nodes represent miRNAs; Triangle nodes represent lncRNAs; Circular nodes represent mRNAs; Every edge indicates target interaction.

Figure 3. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) term enrichment analysis of differentially expressed genes (DEGs). (a) All enriched KEGG pathways of DEGs in ceRNAs network. (b) TOP20 enriched GO biological processes of DEGs in ceRNAs network.

Figure 4. Linear regression of ceRNAs’ expression level. Dashed lines represent 95% confidence interval. (a) ADIRF-AS1 vs MAPK10 (hypertrophic cardiomyopathy, n = 22). (b) ADIRF-AS1 vs SLC6A9 (systemic sclerosis, n = 91). (c) ADIRF-AS1 vs HLF (systemic sclerosis, n = 91). (d) ADIRF-AS1 vs AHNAK2 (systemic sclerosis, n = 91). (e) MBNL1-AS1 vs MYL3 (liver fibrosis, n = 124). (E) MBNL1-AS1 vs POPDC3 (liver fibrosis, n = 124). (f) MBNL1-AS1 vs PLN (liver fibrosis, n = 124).