Long non-coding RNA metastasis-related lung adenocarcinoma transcript 1 (MALAT1) forms a negative feedback loop with long non-coding RNA colorectal neoplasia differentially expressed (CRNDE) in sepsis to regulate lung cell apoptosis

Figures & data

Figure 1. Altered MALAT1 and CRNDE expression levels were observed in sepsis.

MALAT1 (a) and CRNDE (B) levels in plasma samples from both sepsis patients (n = 60) and healthy controls (n = 60) were determined by RT-qPCR. Data are presented as the average of three technical replicates. **, p < 0.01.

Figure 2. MALAT1 was downregulated and CRNDE was upregulated after treatment.

MALAT1 and CRNDE expression levels in plasma samples from sepsis patients at 3 months after treatment were determined by RT-qPCR. Shown are the pre- and post-treatment levels of MALAT1 (a) and CRNDE (b). **, p < 0.01.

Figure 3. MALAT1 and CRNDE negatively regulated each other in HBEpCs.

MALAT1 or CRNDE expression vector was transfected into HBEpCs, and the transfection was confirmed by RT-qPCR at 48 h post-transfection (a). The effects of MALAT1 overexpression on CRNDE (b) and the effects of CRNDE overexpression on MALAT1 (c) were explored using RT-qPCR. Correlations between MALAT1 and CRNDE across plasma samples from both sepsis patients (d) and healthy controls (e) were analyzed using linear regression. Data are shown as the mean ± SD values of three independent replicates. C, control cells without transfection, NC, negative control cells transfected with empty vector. *, p < 0.05.

Figure 4. MALAT1 and CRNDE interacted with each other to regulate LPS-induced HBEpC apoptosis.

HBEpCs were cultured in media supplemented with LPS at doses of 0, 1, 2, 5, and 10 µg/ml for 48 h, and the expression levels of MALAT1 (a) and CRNDE (b) were determined by RT-qPCR. Cell apoptosis assay was performed to analyze the roles of MALAT1 and CRNDE in regulating LPS-induced HBEpC apoptosis (c). The participation of MALAT1 and CRNDE in non-LPS-induced HBEpC apoptosis was explored using cell apoptosis assay (d). Shown are the mean percentages ±SD values of cells positive to both PI/FITC and to PI only from three independent replicates. C, control cells without transfection; NC, negative control cells transfected with empty vector; MALAT1+ CRNDE, cells overexpressed with both MALAT1 and CRNDE. *, p < 0.05.

Data availability statement

The datasets used analyzed during the current study are available from the corresponding author upon reasonable request.