Crosstalk of pyroptosis, ferroptosis, and mitochondrial aldehyde dehydrogenase 2-related mechanisms in sepsis-induced lung injury in a mouse model

Figures & data

Table 1. Comparison of mouse lung coefficients in each group (mean ± SD, n = 6)

Groups	Weight	Lung wet weight	Lung coefficient
Sham	20.20 ± 1.18	104.17 ± 4.83**	5.16 ± 0.14
CLP	21.68 ± 1.78	120.33 ± 5.99*	5.57 ± 0.28*
DMSO+CLP	20.87 ± 1.59	115.50 ± 7.94	5.55 ± 0.13
MCC950+ CLP	21.00 ± 0.71	110.33 ± 4.27^#	5.25 ± 0.11^#
Fer-1+ CLP	20.07 ± 1.23	106.67 ± 6.21^##	5.32 ± 0.20^#
Alda-1+ CLP	21.96 ± 0.97	114.00 ± 8.28^#	5.18 ± 0.19^#

*P < 0.05, **P < 0.01 vs Sham group; ^#P < 0.05, ^##P < 0.01 vs CLP group

Figure 1. Morphological changes after HE staining (a) (200X, Scale bar = 50 μm) and injury scores (b) in mouse lung tissue in different groups (mean ± SD, n = 3), ***P < 0.001 vs sham group; ^##P < 0.01 vs CLP group.

Figure 2. The transmission electron microscopy images of mouse lung tissue (10000x, Scale bar = 2 μm), nucleus (Nu), lamellar bodies (LBs), and mitochondria (Mt).

Figure 3. Detection of MDA (a) and iron (b) in lung tissue of mice (mean ± SD, n = 5), ***P < 0.001 vs sham group; ^#P < 0.05, ^##P < 0.01 vs CLP group.

Figure 4. DHE detection of ROS expression and fluorescence statistics in mouse lung tissue in different groups (200X, Scale bar = 50 μm) a: sham group; b: CLP group; c: MCC950+ CLP group; d: Fer-1+ CLP group; e: Alda-1+ CLP group; f: Fluorescent statistical results (mean ± SD, n = 5), ***P < 0.001 vs sham group; ^##P < 0.01 vs CLP group.

Figure 5. The typical Western blot pictures and statistical results of each group. a, d, g: typical Western blot bands of lung tissue; b, c, e, f, h, i, j, k, l: changes in the expression of different proteins in the lung tissues of each group. (mean ± SD, n = 3), *P < 0.05, **P < 0.01 vs sham group; ^#P < 0.05, ^##P < 0.01 vs CLP group.