miR-139-3p/Kinesin family member 18B axis suppresses malignant progression of gastric cancer

Figures & data

Figure 1. miR-139-3p is poorly expressed in GC. (a) Volcano plot of DEmRNAs and DEmiRNAs in normal and tumor groups. Red: up-regulated genes, green: down-regulated genes; (b) miR-139-3p expression status in normal and tumor samples; (c) Expression status of miR-139-3p in various cell lines. * P< 0.05.

Figure 2. miR-139-3p restrains cell proliferation, migration, invasion, and arrests cell cycle in GC. (a) miR-139-3p level was measured by qRT-PCR; MTT (b), wound healing (c), Tranwell (d) and FCM (e) assays were for cell proliferation, migration and invasion along with cell cycle changes detection, respectively. * P< 0.05.

Figure 3. miR-139-3p can target KIF18B directly. (a) Venn diagram of potential targets of miR-139-3p and up-regulated DEmRNAs, and putative binding sites of miR-139-3p on KIF18B 3ʹUTR; (b) Correlation between KIF18B and miR-139-3p; (c) KIF18B expression in normal and tumor samples; (d) KIF18B mRNA level was measured in GC cell lines; E-F: KIF18B mRNA and protein expression upon overexpression or inhibition of miR-139-3p were tested by qRT-PCR (e) and Western blot (f); (g) The binding relationship of miR-139-3p and KIF18B. * P< 0.05.

Figure 4. KIF18B enhances cell proliferation, migration, invasion, and activates cell cycle of GC. After overexpression or inhibition of KIF18B expression, KIF18B mRNA and protein levels were evaluated by qRT-PCR (a) and Western blot (b). Cell proliferative, migratory, invasive abilities and cell cycle changes were tested by MTT (c), wound healing (d), transwell (e) and FCM (f) assays, respectively. * P< 0.05.

Figure 5. miR-139-3p restrains proliferation, migration, invasion, and arrests cell cycle in GC by regulating KIF18B. With SNU-1 cells transfected with miR-139-3p mimic and oe-KIF18B, KIF18B mRNA and protein expression was assayed by qRT-PCR (a) and Western blot (b). Cell proliferation, migration, invasion and cell cycle changes were tested by MTT (c), wound healing (d), transwell (e) and FCM (f) assays. * represents the comparison with NC mimic group and # represents comparison with miR-139-3p mimic group.

Data availability statement

The data used to support the findings of this study are included within the article. The data and materials in the current study are available from the corresponding author on reasonable request.