Figures & data
Figure 1. circRNA_0000285 serves as a sponge for miR-654-3p. (a) Predicted circRNA_0000285 binding site within the miR-654-3p WT or MUT 3ʹ-UTR. (b) Relative luciferase activity detected following the co-transfection of the luciferase reporter construct containing the miR-654-3p 3ʹ-UTR sequence with the WT or MUT circRNA_0000285 binding site. (c-e) circRNA_0000285-specific probe used to pull-down circRNA_0000285 and enrich miR-654-3p. P < 0.05, **P < 0.01 vs. control.
Figure 2. circRNA_0000258 expression levels are upregulated, while miR-654-3p are down-regulated in CC tissue and cells. (a) Levels of circRNA_0000258 in 30 CC and adjacent normal tissue samples assessed via RT-qPCR. (b) RT-qPCR analysis of circRNA_0000258 in SiHa, HeLa and NC104 cells. (c) Levels of miR-654-3p in 30 CC and adjacent normal tissues evaluated using RT-qPCR. (d) RT-qPCR analysis of miR-654-3p in SiHa, HeLa and NC104 cells analyzed using RT-qPCR. P < 0.05, **P < 0.01 vs. control.
Figure 3. circRNA_0000258 regulates miR-654-3p levels in SiHa and HeLa cells. Expression levels of circRNA_0000258 analyzed in the circ-siRNA and control-siRNA groups in SiHa (a) and HeLa cells (b). Levels of miR-654-3p measured in the miR-654-3p inhibitor and inhibitor control groups in SiHa (c) and HeLa cells (d). (e, f) Levels of miR-654-3p in different groups. P < 0.05, **P < 0.01 vs. control.
Figure 4. Knockdown of circRNA_0000258 regulates SiHa cell viability and apoptosis via upregulation of miR-654-3p expression. SiHa cells were transfected with control-siRNA, circ-siRNA, circ-siRNA + inhibitor control or circ-siRNA + miR-654-3p inhibitor. (a) Effect of circ-siRNA transfection for 48 h on SiHa cell viability evaluated by MTT assay. (b, c) Effect of circ-siRNA on SiHa cell apoptosis analyzed using flow cytometry. (d) Western blotting to detect Bax and Bcl-2 protein expression. (e) Ratio of Bax/Bcl-2. P < 0.05, **P < 0.01 vs. control.
Figure 5. Knockdown of circRNA_0000258 regulates HeLa cell viability and apoptosis via upregulation of miR-654-3p expression. HeLa cells were transfected with control-siRNA, circ-siRNA, inhibitor control or miR-654-3p inhibitor. (a) Cell viability assessed using MTT assay. (b, c) Flow cytometry analysis of apoptotic cells. (d) Expression of Bax and Bcl-2 detected using Western blotting. (e) Ratio of Bax/Bcl-2. P < 0.05, **P < 0.01 vs. control.
Data availability statement
The datasets used and/or analyzed during the present study are available from the correspond