Figures & data
Figure 1. The expression of circ-MYBL2 and miR-19a in PA. Expression analysis of circ-MYBL2 (a) and miR-19a (b) in paired PA and non-tumor tissues from 59 PA patients was performed with RT-qPCR. qPCR was repeated for three times and average values are presented here for comparisons.
Figure 2. Correlation between circ-MYBL2 and miR-19a across PA tissues and non-tumor tissues. Correlations between circ-MYBL2 and miR-19a across PA tissues (a) and non-tumor (b) tissues were subjected to Pearson’s correlation coefficient analysis.
Figure 3. The role of circ-MYBL2 in the expression of miR-19a and its promoter region methylation. SW1990 and PANC-1 cells were overexpressed with circ-MYBL2 or miR-19a, followed by the confirmation of overexpression of circ-MYBL2 and miR-19a by RT-qPCR every 24 h until 96 h (a). Regulatory role of circ-MYBL2 in miR-19a expression (b) and regulatory role of miR-19a in circ-MYBL2 expression (c) were explored by RT-qPCR. The role of overexpression and silencing of circ-MYBL2 in promoter region methylation of miR19a was analyzed with MSP (d). *, p < 0.05.
Figure 4. Analysis of the role of circ-MYBL2 and miR-19a in the proliferation of SW1990 and PANC-1 cells. (a) BrdU assays were performed to analyze the role of circ-MYBL2 and miR-19a in the proliferation of SW1990 and PANC-1 cells. Cell migration (b) and invasion (c) was detected through Transwell assay. The Western blot result of VEGF, MMP2, and MMP9 (d). *, p < 0.05.
