The role of microRNA-4723-5p regulated by c-myc in triple-negative breast cancer

Figures & data

Figure 1. The level of c-myc mRNA detected with real-time PCR after transfection of shRNA in MDA-MB-231 cells. **P < 0.01.

Table 1. The top 10 differentially up-regulated and down-regulated miRNAs

miRNA	log2FoldChange	P	Regulation
hsa-miR-4723-5p	−2.90660322205891	0.020705437	down
hsa-miR-4742-5p	−2.43620880088277	0.005744107	down
hsa-miR-3681-5p	−2.37910283430097	8.36E-32	down
hsa-miR-505-5p	−2.2418462837511	0.000370238	down
hsa-miR-935	−2.19336093592009	0.001517733	down
hsa-miR-4662a-5p	−2.01907675440698	0.008739171	down
hsa-miR-196a-5p	−2.0030557180194	4.15E-05	down
hsa-miR-1284	−1.96189932602249	0.01587404	down
hsa-miR-5010-5p	−1.94732231485239	0.037681789	down
hsa-miR-4688	−1.89600708714419	0.045625698	down

Figure 2. Heat map of upregulated and downregulated differentially expressed miRNAs after knocking down c-myc. Each column represents a sample, and each row represents a differential miRNA. Red indicates high expression, and green indicates low expression.

Figure 3. The top-10 enriched GO terms of target genes of differentially expressed miRNAs. The ordinate is the number of genes enriched by GO, and the abscissa is the name of the GO term.

Figure 4. KEGG pathway enrichment bubble chart of top-20 enriched pathways of target genes of differentially expressed miRNAs. Each dot corresponds to a pathway. The color gradient, from red to purple, corresponds to the P value. The lower the P value, the more the color tends toward red. The larger the dot, the more genes in the pathway.

Table 2. Some representative target genes of hsa-miR-4723-5p

	Predicted target genes using miRDB database	Predicted target genes using miWalk database	Common target genes predicted by two databases
miR-4723-5p	PDX1	PDX1	PDX1
	PAX2	PAX2	PAX2
	POLR2A	POLR2A	POLR2A
	ATN1	ATN1	ATN1
	FOXP4	FOXP4	FOXP4
	MINK1	MINK1	MINK1

Table 3. The top 8 enriched GO terms and KEGG pathways of target genes of hsa-miR-4723-5p

Category	Term	P	Gene
Biological process
GO:0031573	intra-S DNA damage checkpoint	7.41E-66	78
GO:0035025	positive regulation of Rho protein signal transduction	4.82E-59	79
GO:0036309	protein localization to M-band	1.30E-56	59
GO:0021801	cerebral cortex radial glia guided migration	2.00E-54	59
GO:0010573	vascular endothelial growth factor production	2.00E-54	58
GO:0090286	cytoskeletal anchoring at nuclear membrane	2.15E-51	61
GO:0030048	actin filament-based movement	1.08E-49	67
GO:0030534	adult behavior	1.50E-49	93
Cellular component
GO:0031430	M band	7.51E-70	96
GO:0030018	Z disc	1.24E-61	185
GO:0030016	myofibril	1.10E-59	91
GO:0097451	glial limiting end-foot	2.07E-58	42
GO:0034993	LINC complex	7.30E-54	48
GO:0005592	collagen type XI trimer	3.46E-48	32
GO:0005694	chromosome	1.21E-43	128
GO:0000118	histone deacetylase complex	3.82E-41	84
Molecular function
GO:0051015	actin filament binding	8.96E-91	196
GO:0030506	ankyrin binding	1.26E-84	103
GO:0003779	actin binding	9.42E-74	299
GO:0008307	structural constituent of muscle	3.71E-70	117
GO:0008022	protein C-terminus binding	2.31E-46	176
GO:0000146	microfilament motor activity	7.74E-43	56
GO:0005516	calmodulin binding	7.28E-42	162
GO:0050840	extracellular matrix binding	7.10E-39	58
KEGG pathway
hsa04974	Protein digestion and absorption	1.44E-45	89
hsa04713	Circadian entrainment	2.19E-16	65
hsa04512	ECM-receptor interaction	7.19E-15	54
hsa00310	Lysine degradation	1.32E-13	42
hsa04724	Glutamatergic synapse	1.48E-10	52
hsa00750	Vitamin B6 metabolism	7.43E-10	9
hsa04930	Type II diabetes mellitus	1.19E-09	34
hsa04530	Tight junction	8.71E-09	56

Figure 5. The expression levels of miR-4723-5p verified by real-time PCR. The expression level of miR-4723-5p. *P < 0.05, **P < 0.01.