Long intergenic non-protein coding RNA 00707 regulates chondrocyte apoptosis and proliferation in osteoarthritis by serving as a sponge for microRNA-199-3p

Figures & data

Table 1. Clinical features of OA and Control groups

	OA (I = 38)	Control (n = 38)
Gender (Female)	27	27
Age (Years, mean ± SD)	56.72 ± 7.49	56.23 ± 8.24
Obesity %	12	10
Disease duration (months ± SD)	62,12 ± 12.34	NA
Kellgren–Lawrence stage
III	14	NA
IV	24	NA
Smokers (%)	16	17
Drinkers (%)	23	21

Figure 1. RT-qPCR analysis of the expression of LINC00707 and miR-199-3p in OA.

Figure 2. Subcellular location of LINC00707 in chondrocytes and analysis of its interaction with miR-199-3p. To analyze the subcellular location of LINC00707, nuclear fractionation assay was performed on chondrocytes (a). The interaction between LINC00707 and miR-199-3p was predicted by IntaRNA 2.0 (b). The direct interaction between LINC00707 and miR-199-3p was analyzed by RNA pull-down assay (c). Dual-luciferase reporter assay was performed by transfecting LINC00707 and miRNA NC or LINC00707 and miR-199-3p into chondrocytes (d). *p < 0.05; ***p < 0.001.

Figure 3. Analysis of the regulator roles of LINC00707 and miR-199-3p in the expression of each other. The direct interaction may indicate the regulator roles of LINC00707 and miR-199-3p in the expression of each other. Pearson’s correlation coefficient analysis was done to analyze the correlations between LINC00707 and miR-199-3p across OA (a) and control (b) samples. Chondrocytes were overexpressed with LINC00707 or miR-199-3p, and the overexpression was confirmed by RT-qPCR every 24 h until 72 h (c). The role of LINC00707 in the expression of miR-199-3p (d), and the role of miR-199-3p in the expression of LINC00707 (e) were analyzed by RT-qPCR. *p < 0.05.

Figure 4. Analysis of the roles of LINC00707 and miR-199-3p in the proliferation and apoptosis of chondrocytes. The roles of LINC00707 and miR-199-3p in the proliferation and apoptosis (induced by 10 μg/ml LPS for 24 h) of chondrocytes were analyzed by BrdU assay (a) and cell apoptosis assay (b), respectively. *p < 0.05.

Data availability statement

The datasets generated and/or analyzed during the current study are available in https://115.com/s/swnywtq3zyl?password=u576&#, the code is u576.