Figures & data
Figure 1. PVT1 and GAS5 expression alterations were observed in OA synovial fluid cells. PVT1 (a) and GAS5 (b) accumulation in synovial fluid samples from both OA patients (n = 62) and controls (n = 62) and expression of PVT1 (c) and GAS5 (d) in synovial tissue samples from both OA patients (n = 6) and controls (n = 4) were determined by RT-qPCR. The three lines represent 25%, median, and 75% values, respectively. *: p < 0.05; **: p < 0.01.
Figure 2. PVT1 and GAS5 were inversely correlated only in OA patients but not in healthy controls. The correlations of PVT1 to GAS5 across OA (a) and control (b) synovial fluid samples were studied with linear regression.
Figure 3. Regulatory role of LPS in PVT1 and GAS5 accumulation in chondrocytes. Chondrocytes were cultured in media supplemented with LPS (0, 1, 2, 4, 8, and 12 ng/ml) for 48 h, followed by determination of PVT1 and GAS5 in both chondrocytes (a) and cell culture medium (b). *: p < 0.05.
Figure 4. PVT1 and GAS5 negatively regulate each other in chondrocytes. Chondrocytes overexpressed PVT1 or GAS5 (a). The regulatory role of PVT1 in GAS5 accumulation (b) and the regulatory role of GAS5 in PVT1 accumulation (c) were studied with RT-qPCR. Dual-luciferase reporter assay was performed to analyze the binding of PVT1 and GAS5 to each other’s promoter regions (d). *: p < 0.05.
Figure 5. The negative feedback loop formed by PVT1 and GAS5 regulates chondrocyte apoptosis induced by LPS. The roles of PVT1 and GAS5 in regulating chondrocyte apoptosis induced by LPS (12 ng/ml) were explored by cell apoptosis assay. *: p < 0.05.
Figure 6. The negative feedback loop formed by PVT1 and GAS5 regulates chondrocyte apoptosis relative protein expression induced by LPS. The roles of PVT1 and GAS5 in regulating Cas-3 and Bax expression induced by LPS (12 ng/ml) were explored by western blot assay. *: p < 0.05.
