Figures & data
Figure 1. Sufentanil decreased the viability of lung cancer cells. (a) The molecular formula of sufentanil. (b) The cell viability of the H460 and H1299 was detected by MTT assay after sufentanil (0.5, 1, 2 nM) treatment. (c) The cell viability of the H460 and H1299 was detected by MTT assay at 12, 24, 48 h, after 2 nM sufentanil treatment.
Figure 2. Sufentanil decreased the proliferation, migration, and invasion abilities of the lung cancer cells. (a-b) The cell proliferation of the H460 and H1299 cells was measured by colony formation and EdU assays after 2 nM sufentanil treatment. (c-d) The migration and invasion abilities of the H460 and H1299 cells were tested by transwell assay after 2 nM sufentanil treatment. (e) The protein expressions of Vimentin, N-cadherin, E-cadherin and ZO-1 were measured by western blot after 2 nM sufentanil treatment.
Figure 3. Sufentanil inhibited the Wnt/β-catenin signaling pathway. (a-b) The protein expressions of β-catenin, c-Myc, and MMP2 of the H460 and H1299 cells were measured by western blot after 2 nM sufentanil treatment.
Figure 4. LiCl activated the Wnt/β-catenin signaling pathway. (a-b) The protein expressions of β-catenin, c-Myc, and MMP2 of the H460 and H1299 cells were measured by western blot after 2 nM sufentanil and 10 mM LiCl treatment.
Figure 5. Activation of the Wnt/β-catenin signaling pathway reversed the sufentanil effects. (a-b) The cell proliferation of the H460 and H1299 cells was measured by colony formation and EdU assays after 2 nM sufentanil and 10 mM LiCl treatment. (c-d) The migration and invasion abilities of the H460 and H1299 cells were tested by transwell assay after 2 nM sufentanil and 10 mM LiCl treatment. € The protein expressions of Vimentin, N-cadherin, E-cadherin and ZO-1 were measured by western blot after 2 nM sufentanil treatment.
Data Availability Statement
The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.