Suppression of long intergenic non-protein coding RNA 1123 constrains lower extremity deep vein thrombosis via microRNA-125a-3p to target interleukin 1 receptor type 1

Figures & data

Table 1. Primer sequences

Genes	Sequences
LINC01123	F: 5’-TTTTGGTTTGAGGGCATAGGG-3’
	R: 5’-CAACAGCATTGACGAGACACATTT-3’
MiR-125a-3p	F: 5’-TGACACAGGTGAGGTTCTTG-3’
	R: 5’-TATGGTTTTGACGACTGTGTGAT-3’
IL1R1	F: 5’-GTGCTACTGGGGCTCATTTGT-3’
	R: 5’-GGAGTAAGAGGACACTTGCGAAT-3’
U6	F: 5’-CGCTTCGGCAGCACATATAC-3’
	R: 5’- AAATATGGAACGCT-TCACGA-3’
GAPDH	F: 5’- ACCCAGAAGACTGTGGATGG-3’
	R: 5’- GGAGACAACCTGGTCCTCAG-3’

Figure 1. LINC01123 and IL1R1 are elevated while miR-125a-3p is silenced in LEDVT rats. (a): HE staining assessment of pathological conditions; (b): Masson staining evaluation of pathological conditions; (c-d): Length and weight of thrombus; (e-f): Inflammatory cytokines IL-6 and IL-8 in the serum; (g-h): RT-qPCR or Western Blot examination of LINC01123, miR-125a-3p and IL1R1. Values are expressed as mean ± standard deviation (n = 6). * Vs. the Sham, P < 0.05.

Figure 2. Silence of LINC01123 represses inflammation and thrombosis in LEDVT rats. (a): RT-qPCR test of LINC01123; (b-c): Length and weight of thrombus; (d-e): Inflammatory cytokines IL-6 and IL-8 in the serum; (f): HE staining assessment of pathological conditions; (g): Masson staining evaluation of pathological conditions; Values are expressed as mean ± standard deviation (n = 6). * Vs. the sh-NC, P < 0.05.

Figure 3. LINC01123 modulates IL1R1 via miR-125a-3p. (a-b): Bioinformation website prediction of the targeted binding site of miR-125a-3p with LINC01123 or IL1R1; (c-d): The luciferase activity assay verification of the targeting of miR-125a-3p with LINC01123 or IL1R1; E-F: RT-qPCR or Western Blot detection of miR-125a-3p with IL1R1. Values are expressed as mean ± standard deviation (n = 6), cell experiments were repeated three times. * Vs. the sh-NC, P < 0.05.

Figure 4. Elevated miR-125a-3p represses inflammation and thrombosis in LEDVT rats. (a): RT-PCR test of miR-125A-3p; (b-c): Length and weight of thrombus; (d-e): Inflammatory cytokines IL-6 and IL-8 in the serum; (f): HE staining assessment of pathological conditions; (g): Masson staining evaluation of pathological conditions; Values are expressed as mean ± standard deviation (n = 6). * Vs. the agomir NC group, P < 0.05.

Figure 5. Elevated IL1R1 turns around the action of repressive LINC01123 on LEDVT rats. (a): RT-qPCR and Western Blot verification of successful injection; (b-c): Length and weight of thrombus; (d-e): Inflammatory cytokines IL-6 and IL-8 in the serum; F: HE staining assessment of pathological conditions; (g): Masson staining evaluation of pathological conditions; Values are expressed as mean ± standard deviation (n = 6). * Vs. the sh-LINC01123 + oe-NC, P < 0.05.