Circular RNA hsa_circ_0000437 may be used as a new indicator for the diagnosis and prognosis of hepatocellular carcinoma

Figures & data

Table 1. Correlation between hsa_circ_0000437 expression and clinical parameters in HCC.

Clinicopathologic characteristics	Tissue				Serum
	n	Low (n = 32)	High (n = 68)	p value	n	Low (n = 44)	High (n = 56)	p value
Age (years)				0.7444				0.7970
< 55	23	8	15		26	12	14
≥ 55	76	24	53		74	32	42
Sex				0.3733				0.7917
Male	83	25	58		76	34	42
Female	17	7	10		24	10	14
TNM stage				0.0215*				0.0342*
I+II	52	22	30		54	29	25
III+IV	48	10	38		46	15	31
HCC history				0.0788				0.3690
Positive	71	19	52		68	32	36
Negative	29	13	16		32	12	20
Differentiation				0.0353*				0.0348*
High/moderate	73	19	54		70	26	44
Low	27	13	14		30	18	12
Tumor size				0.0322*				0.0195*
< 50 mm	81	22	59		77	29	48
≥ 50 mm	19	10	9		23	15	8
Tumor number				0.7679				0.1510
Single	67	21	46		65	32	33
Multiple	33	11	22		35	12	23
BCLC stage				0.0371*				0.0258*
A + B	88	25	63		86	34	52
C + D	12	7	5		14	10	4
HbsAg				0.2293				0.2624
Negative	23	5	18		26	9	17
Positive	77	27	50		74	35	39

Figure 1. Aberrantly expressed circRNAs identified in GSE166678 associated with HCC. A. Heat map of abnormally expressed circRNAs. B. Volcano map of abnormally expressed circRNAs. C. RT-qPCR analyses of circ_0008092, circ_0097182, circ_0000437, circ_0028071, and circ_0036409 expression in HCC tissues and healthy controls. *P< 0.05, **P< 0.01, ***P< 0.001.

Figure 2. Expression of circ_0000437 in the tissues and serum of the normal control and tumor groups. ***P< 0.001.

Table 2. Relationship between the expression of hsa_circ_0000437 in serum and clinical characteristics of enrolled HCC patients.

Factors	Regression coefficient β	OR (95% CI)	p value
Age (years, < 55 vs. ≥ 55)	−0.03	0.971 (0.546, 1.727)	0.919
Gender (Male vs. Female)	−0.261	0.77 (0.401, 1.478)	0.423
TNM stage (I +II vs. III+ IV)	1.788	5.976 (2.77, 12.894)	<0.0001*
HCC history (Positive vs. Negative)	−0.355	0.701 (0.401, 1.226)	0.213
Differentiation (High/moderate vs. Low)	0.964	2.623 (1.39, 4.95)	0.003*
Tumor size (<50 mm vs. ≥ 50 mm)	0.761	2.141 (1.096, 4.185)	0.026*
Tumor number (Single vs. Multiple)	0.388	1.474 (0.867, 2.505)	0.152
BCLC stage (A + B vs. C + D)	0.75	2.118 (0.953, 4.705)	0.065
HbsAg (Negative vs. Positive)	0.437	1.547 (0.853, 2.806)	0.151

Figure 3. OS and ROC curves of circ_0000437 in HCC.

Data availability statement

The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.