Figures & data
Figure 1. The effect of Ibuprofen on p75NTR expression in SGC7901 and MKN45 cells. (a) The qPCR results of p75NTR in SGC7901 and MKN45 cells. (b-c) Protein expression of p75NTR was determined using Western blot. Results are represented as mean fold-change ± SD (n = 3). Statistical significance was determined by one-way ANOVA with the Bonferroni multiple comparisons test (*p < 0.05, **p < 0.01, ***p < 0.001).
Figure 2. The effect of Ibuprofen on the promoter methylation and m6A-p75NTR in SGC7901 and MKN45 cells. (a) The OneStep qMethyl Kit results of p75NTR in SGC7901 and MKN45 cells. (b) RNA immunoprecipitation using m6A antibody and quantification of p75NTR mRNA levels in control and Ibuprofen-treated gastric cancer cells. Results are represented as mean fold-change ± SD (n = 3). Statistical significance was determined by one-way ANOVA with the Bonferroni multiple comparisons test (***p < 0.001).
Figure 3. The effect of Ibuprofen on the expression of m6A methyltransferases and demethylases in SGC7901 and MKN45 cells. The qPCR results of METTL3 (a), METTL14 (b), ALKBH5 (c) and FTO (d) in SGC7901 and MKN45 cells. Results are represented as mean fold-change ± SD (n = 3). Statistical significance was determined by one-way ANOVA with the Bonferroni multiple comparisons test (*p < 0.05, ***p < 0.001).
Figure 4. The effect of Ibuprofen on the expression of m6A readers in SGC7901 and MKN45 cells. The qPCR results of YTHDC2 (a), YTHDF1 (b), YTHDF2 (c), and YTHDF2 (d) in SGC7901 and MKN45 cells. Results are represented as mean fold-change ± SD (n = 3). Statistical significance was determined by one-way ANOVA with the Bonferroni multiple comparisons test (***p < 0.001).
Supplemental Material
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All data and information can be obtained from the corresponding author on reasonable request.
