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Addendum

Pigment epithelium-derived factor (PEDF) normalizes matrix defects in iPSCs derived from Osteogenesis imperfecta Type VI

, &
Article: e1212150 | Received 18 May 2016, Accepted 06 Jul 2016, Published online: 10 Aug 2016

Figures & data

Figure 1. Wildtype (WT) or OI6 iPSC-derived MSCs were differentiated for 21 d in osteogenic media. (A) Col1A1 qPCR on WT and 3 OI6 iPSC-derived clones. (B) IBSP qPCR on WT and 3 OI6 iPSC-derived clones. (C) IBSP expression on WT and clone1 with PEDF treatment. (D) Immunoblots for IBSP protein from WT or OI6 clone 1 cells. Where indicated, 300 ng/ml PEDF was added to the culture media on days 14–21. error bars = SEM, 3–6 biological replicates per group.

Figure 1. Wildtype (WT) or OI6 iPSC-derived MSCs were differentiated for 21 d in osteogenic media. (A) Col1A1 qPCR on WT and 3 OI6 iPSC-derived clones. (B) IBSP qPCR on WT and 3 OI6 iPSC-derived clones. (C) IBSP expression on WT and clone1 with PEDF treatment. (D) Immunoblots for IBSP protein from WT or OI6 clone 1 cells. Where indicated, 300 ng/ml PEDF was added to the culture media on days 14–21. error bars = SEM, 3–6 biological replicates per group.