Preparation of imprinted cryogel cartridge for chiral separation of l-phenylalanine

Figures & data

Table 1. The combination ratios of the template and functional monomer.

	l-Phe:MAPA
Polymer code	Molar ratio (mol:mol)	Mass ratio
PHEMAPA-l-Phe (A)	1:1	14 mg/19.9 mg
PHEMAPA-l-Phe (B)	1:2	14 mg/39.8 mg
PHEMAPA-l-Phe (C)	1:3	14 mg/59.7 mg

Figure 1. SEM images of (A) PHEMAPA (B) PHEMAPA-l-Phe cryogels.

Figure 2. The effect of adsorption conditions on l-Phe adsorption onto PHEMAPA-l-Phe cryogel membranes. (A) Effect of pH (l-Phe concentration: 0.5 mg/mL; flow rate: 1 mL/min; adsorption time: 2 h; T: 25 °C) (B) Effect of flow rate (l-Phe concentration: 1.0 mg/mL; pH: 5.5; adsorption time: 2 h; T: 25 °C). (C) Effect of l-Phe concentration (pH: 5.5; flow rate: 1.0 mL/min; adsorption time: 2 h; T: 25 °C) (D) Effect of temperature (l-Phe concentration: 1.5 mg/mL; pH: 5.5; adsorption time: 2 h) (E) Effect of ionic strength (l-Phe concentration: 1.5 mg/mL; pH: 5.5; adsorption time: 2 h; T: 25 °C) (F) Effect of salt type (l-Phe concentration: 1.5 mg/mL; salt concentration: 0.1 M; pH: 5.5; adsorption time: 2 h; T: 25 °C).

Table 2. The pseudo-first-order and pseudo-second-order kinetic constants for l-Phe adsorption.

	Experimental	Pseudo 1°			Pseudo 2°
l-Phe (mg/mL)	qeq (mg/g)	k1	qeq	R^2	k2	qeq	R^2
1.5	24.1	0.133	166.4	0.8	6.08x10^−4	36.1	0.904

Figure 3. Adsorption of competitor amino acids on PHEMAPA-l-Phe and PHEMAPA cryogels. Amino acid concentration: 1.0 mg/mL; pH: 5.5; adsorption time: 2 h; T: 25 °C.

Table 3. K_d and k values for d-Phe, l-Trp, and d-Trp with respect to l-Phe.

	PHEMAPA		PHEMAPA-l-Phe
Molecule	Kd	k	Kd	k	k'
l-Phe	4.78	–	28.63	–	–
d-Phe	3.99	1.20	3.75	7.63	6.36
l-Trp	0.72	6.63	1.00	28.42	4.29
d-Trp	2.18	2.20	2.37	12.07	5.49

Figure 4. FPLC separation of d-Phe and l-Phe with MIP column.

Table 4. The parameters calculated from the purification chromatograms.

	tR	N	k	α
Unbound	1.70	191	–	–
d-Phe	13.50	29,696	45.55	–
l-Phe	14.10	35,290	47.62	1.05