1,893
Views
17
CrossRef citations to date
0
Altmetric
Articles

Development of a novel biosensor based on a polypyrrole–dodecylbenzene sulphonate (PPy–DBS) film for the determination of amperometric cholesterol

&
Pages 824-832 | Received 01 Mar 2016, Accepted 10 Apr 2016, Published online: 30 Aug 2016

Figures & data

Figure 1. Growth of PPy–DBS films in aqueous solution of 0.15 M pyrrole and 0.2 M DBS at a scan rate of 20 mV/s vs Ag/AgCl electrode (3.0 M KCl).

Figure 1. Growth of PPy–DBS films in aqueous solution of 0.15 M pyrrole and 0.2 M DBS at a scan rate of 20 mV/s vs Ag/AgCl electrode (3.0 M KCl).

Figure 2. The SEM of Pt/PPy–DBS electrode.

Figure 2. The SEM of Pt/PPy–DBS electrode.

Figure 3. The SEM of Pt/PPy–DBS–ChOx.

Figure 3. The SEM of Pt/PPy–DBS–ChOx.

Figure 4. The AFM of Pt/PPy–DBS–ChOx.

Figure 4. The AFM of Pt/PPy–DBS–ChOx.

Figure 5. The AFM of Pt/PPy–DBS electrode.

Figure 5. The AFM of Pt/PPy–DBS electrode.

Figure 6. The contact angle of Pt/PPy–DBS electrode.

Figure 6. The contact angle of Pt/PPy–DBS electrode.

Figure 7. The contact angle of Pt/PPy–DBS–ChOx.

Figure 7. The contact angle of Pt/PPy–DBS–ChOx.

Figure 8. Amperometric response measurements of Pt/PPy–DBS electrode.

Figure 8. Amperometric response measurements of Pt/PPy–DBS electrode.

Figure 9. The effects of different potentials on amperometric response measurements.

Figure 9. The effects of different potentials on amperometric response measurements.

Figure 10. The effects of different pyrrole concentrations on amperometric response measurements.

Figure 10. The effects of different pyrrole concentrations on amperometric response measurements.

Figure 11. The effects of different concentrations of sodium dodecylbenzene sulphonate on amperometric response measurements.

Figure 11. The effects of different concentrations of sodium dodecylbenzene sulphonate on amperometric response measurements.

Figure 12. The effects of cycle number of polypyrrole film on amperometric response measurements.

Figure 12. The effects of cycle number of polypyrrole film on amperometric response measurements.

Figure 13. Amperometric response measurements of Pt/PPy–DBS–ChOx electrode.

Figure 13. Amperometric response measurements of Pt/PPy–DBS–ChOx electrode.

Figure 14. The effect of pH on the response of the biosensor (in the phosphate buffer (pH 7.0) containing 50 μM cholesterol at 0.4 V working potential).

Figure 14. The effect of pH on the response of the biosensor (in the phosphate buffer (pH 7.0) containing 50 μM cholesterol at 0.4 V working potential).

Figure 15. The effect of temperature on the response of the biosensor (at pH 7.0, 50 μM cholesterol at 0.4 V working potential).

Figure 15. The effect of temperature on the response of the biosensor (at pH 7.0, 50 μM cholesterol at 0.4 V working potential).

Figure 16. The effect of cholesterol concentration on the response of the biosensor (at 0.1 M pH 7.0 the phosphate buffer, 25 °C, 0.4 V working potential).

Figure 16. The effect of cholesterol concentration on the response of the biosensor (at 0.1 M pH 7.0 the phosphate buffer, 25 °C, 0.4 V working potential).

Figure 17. The effect of cholesterol concentration on the response of the biosensor (Lineweaver–Burk plot at 0.1 M pH 7.0 phosphate buffer, 25 °C, 0.4 V working potential).

Figure 17. The effect of cholesterol concentration on the response of the biosensor (Lineweaver–Burk plot at 0.1 M pH 7.0 phosphate buffer, 25 °C, 0.4 V working potential).

Figure 18. Operational stability of the biosensor in pH 7.0 phosphate buffer, 50 μM cholesterol 25 °C, 0.4 V working potential.

Figure 18. Operational stability of the biosensor in pH 7.0 phosphate buffer, 50 μM cholesterol 25 °C, 0.4 V working potential.

Figure 19. Storage stability of the biosensor in pH 7.0 phosphate buffer, 50 μM cholesterol 25 °C, 0.4 V working potential.

Figure 19. Storage stability of the biosensor in pH 7.0 phosphate buffer, 50 μM cholesterol 25 °C, 0.4 V working potential.

Table 1. Interfering substances on the amperometric response of the biosensor.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.