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Original Article

Polychelated cryogels: hemoglobin adsorption from human blood

Pages 31-38 | Received 18 May 2016, Accepted 18 Jun 2016, Published online: 10 Aug 2016

Figures & data

Figure 1. Schematic representation of poly(HEMA-GMA)-PEI-Cu(II) synthesis.

Figure 1. Schematic representation of poly(HEMA-GMA)-PEI-Cu(II) synthesis.

Figure 2. The SEM image of poly(HEMA-GMA)-PEI-Cu(II) cryogels.

Figure 2. The SEM image of poly(HEMA-GMA)-PEI-Cu(II) cryogels.

Figure 3. The FT-IR spectra of (a) Poly(HEMA-GMA), (b) Poly(HEMA-GMA)-PEI, (c) Poly(HEMA-GMA)-PEI-Cu(II) cryogels.

Figure 3. The FT-IR spectra of (a) Poly(HEMA-GMA), (b) Poly(HEMA-GMA)-PEI, (c) Poly(HEMA-GMA)-PEI-Cu(II) cryogels.

Figure 4. The effect of (a) pH. CHemoglobin: 1 mg/mL; interaction time: 30 min.; temperature: 25 °C. (b) Interaction time. CHemoglobin: 1 mg/mL; pH:6.0; temperature: 25 °C. (c) Initial hemoglobin concentration. pH:6.0; interaction time: 30 min.; temperature: 25 °C. (d) Ionic strength. pH:6.0; CHemoglobin: 1 mg/mL; interaction time: 30 min.; temperature: 25 °C. (e) Temperature. pH:6.0; CHemoglobin: 1 mg/mL; interaction time: 30 min., on the hemoglobin adsorption. All experiments were repeated three times with triplicated measurements while applying 95% confidence interval for calculating mean values reported.

Figure 4. The effect of (a) pH. CHemoglobin: 1 mg/mL; interaction time: 30 min.; temperature: 25 °C. (b) Interaction time. CHemoglobin: 1 mg/mL; pH:6.0; temperature: 25 °C. (c) Initial hemoglobin concentration. pH:6.0; interaction time: 30 min.; temperature: 25 °C. (d) Ionic strength. pH:6.0; CHemoglobin: 1 mg/mL; interaction time: 30 min.; temperature: 25 °C. (e) Temperature. pH:6.0; CHemoglobin: 1 mg/mL; interaction time: 30 min., on the hemoglobin adsorption. All experiments were repeated three times with triplicated measurements while applying 95% confidence interval for calculating mean values reported.

Figure 5. The reusability of poly(HEMA-GMA)-PEI-Cu(II) cryogels.

Figure 5. The reusability of poly(HEMA-GMA)-PEI-Cu(II) cryogels.

Figure 6. The hemoglobin adsorption performance comparison of poly(HEMA-GMA), poly(HEMA-GMA)-PEI and poly(HEMA-GMA)-PEI-Cu(II) cryogels.

Figure 6. The hemoglobin adsorption performance comparison of poly(HEMA-GMA), poly(HEMA-GMA)-PEI and poly(HEMA-GMA)-PEI-Cu(II) cryogels.

Figure 7. The UV spectrum of hemoglobin desorbed.

Figure 7. The UV spectrum of hemoglobin desorbed.

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