HEMO₂life as a protective additive to Celsior solution for static storage of donor hearts prior to transplantation

Figures & data

Figure 1. Study protocol for isolated Langendorff heart perfusion groups. Hearts were subjected to an initial equilibration period of 20 min (when pre-ischemic function was measured), arrested with a 2-min infusion (at 4–8 °C) of the cardioplegic solution prior to 8 h of cold (4–8 °C) storage in Celsior supplemented (treated group, n = 12) or not (control group, n = 12) with 1 g/L of HEMO₂life^® (M101) and then reperfused for 60 min with warm (37 °C) Krebs–Henseleit bicarbonate buffer. Recovery of function (expressed as a % of pre-ischemic function) was assessed throughout reperfusion.

Figure 2. Recovery of LVDP (% pre-ischemic function) over 60 min reperfusion with warm (37 °C) Krebs–Henseleit bicarbonate buffer in C and C + M101 groups. *P < 0.0001 versus Celsior.

Table 1. Celsior versus Celsior + M101: baseline data for each function parameter (LVDP, LVEDP, HR and CF), and recovery data during 60 min of reperfusion.

	Celsior				Celsior + M101
n = 12 hearts	LVDP (mmHg)	LVEDP (mmHg)	HR (bpm)	CF (ml/min)	LVDP (mmHg)	LVEDP (mmHg)	HR (bpm)	CF (ml/min)
Baseline	140.9 ± 3.2	3.8 ± 0.5	251.3 ± 9.4	12.4 ± 0.6	131.3 ± 5.0	4.0 ± 0.4	243.0 ± 10.0	12.5 ± 1.0
Reperfusion time (min)	LVDP (% of baseline)	LVEDP (mmHg)	HR (% of baseline)	CF (ml/min)	LVDP (% of baseline)	LVEDP (mmHg)	HR (% of baseline)	CF (ml/min)
0	0	9.7 ± 2.0	0	NA	0	9.3 ± 1.9	0	NA
5	42.2 ± 8.9	57.8 ± 7.5	66.5 ± 8.6	NA	40.6 ± 7.1	60.7 ± 4.2	72.6 ± 7.7	NA
10	37.8 ± 9.5	57.1 ± 8.1	60.9 ± 9.3	NA	40.2 ± 5.7	57.7 ± 4.2	68.4 ± 4.7	NA
20	50.0 ± 5.8	44.2 ± 6.2	88.7 ± 4.4	8.8 ± 0.6	56.5 ± 4.2	42.8 ± 3.1	85.4 ± 7.2	10.2 ± 0.9
30	51.2 ± 2.9	38.2 ± 5.2	88.7 ± 3.7	7.2 ± 0.5	62.6 ± 2.7*	36.7 ± 2.7	90.5 ± 7.6	9.4 ± 0.7**
40	48.7 ± 1.8	36.4 ± 4.8	85.5 ± 3.6	6.4 ± 0.5	61.7 ± 1.9*	33.8 ± 2.6	91.4 ± 7.2	8.5 ± 0.7**
50	47.2 ± 1.9	35.4 ± 4.5	84.0 ± 3.3	5.8 ± 0.4	59.4 ± 1.6*	31.8 ± 2.6	91.1 ± 8.0	7.9 ± 0.5**
60	44.7 ± 2.0	34.8 ± 4.6	84.0 ± 3.8	5.4 ± 0.4	57.3 ± 1.3*	31.1 ± 2.6	88.6 ± 7.4	7.5 ± 0.7**

*P < 0.0001 versus Celsior; **P < 0.02 versus Celsior. NA: not applicable.

Figure 3. Recovery of CF in C and C + M101 groups. CF was measured from t = 20 min. *P < 0.02 versus Celsior.

Figure 4. Recovery of (A) LVEDP and (B) HR for C and C + M101.

Figure 5. Myocardial viability (TTC staining) for C and C + M101 (A) and Representative transverse heart sections (2 mm) from C (a), C + M101 hearts (b), stained with TTC at the end of the isolated heart perfusion protocol. Very patchy areas of pale yellow in the myocardium are visible, representing non-viable tissues (B).