Silibinin-loaded magnetic nanoparticles inhibit hTERT gene expression and proliferation of lung cancer cells

Figures & data

Table 1. hTERT mRNA level in Real-time PCR measurement.

Silibinin-loaded PLGA-PEG-Fe3O4	CT hTERT	Ct reference	Δ ct	2ΔΔct
Untreated	19.20	21.60	2.43	1.000
100 μM	21.09	20.99	0.22	0.216
80 μM	21.39	22.14	0.8	0.323
60 μM	21.75	22.98	1.49	0.521
40 μM	24.68	21.85	2.83	0.757
Silibinin With nanoparticle treated (60 μM)	28.40	21.72	6.68	0.05
Silibinin With nanoparticle treated (40 μM)	21.40	21.05	.35	0.236
Silibinin With nanoparticle treated (20 μM)	25.18	21.26	3.92	0.356

Figure 1. Scanning electron microscopy of (A) Fe₃O₄ magnetic nanoparticles, and (B) silibinin-loaded Fe₃O₄ magnetic nanoparticles modified with PLGA-PEG copolymers.

Figure 2. FTIR plot of (A) PLGA-PEG copolymer nanoparticles, (B) pure Fe₃O₄ nanoparticles and (C) loading silibinin in nanoparticle PLGA-PEG-Fe₃O₄.

Figure 3. ¹HNMR of PLGA-PEG copolymer.

Figure 4. Magnetic hysteresis curve. (A) Magnetic hysteresis curve of pure Fe₃O₄ nanoparticles and (B) magnetic hysteresis curve of silibinin-loaded PLGA-PEG-Fe₃O₄ nanoparticles.

Figure 5. Cytotoxic effect of different concentrations of silibinin on A549 lung cancer cell line during 24 (A), 48 (B), and 72(C) h.

Figure 6. Result of hTERT gene expression study by real-time PCR. Effect of different concentrations of pure silibinin and silibinin-loaded PLGA-PEG-Fe₃O₄ nanoparticles on the levels of hTERT genes expressions in A549 lung cancer cell line.