http://orcid.org/0000-0002-8391-9228
Figures & data
Table 1. Mice immunization regimen by different formulations.
Figure 1. FTIR spectrum of G1 and G2 dendrimers (1a) and NMR characterization of conjugated dendrimer with polytope (1b).
Figure 2. Analysis of humoral responses, total IgG (2a) and IgG2a/IgG1 ratio (2b) in mice immunized with candidate vaccines in different formulations by ELISA. The asterisks directly above the bars point out statistically significant differences between conjugated immunized groups and the polytope-administrated group of mice.
Figure 3. IFN-γ and IL4 cytokine production levels. To characterize antigen-specific, cell-mediated responses, splenic mononuclear cells were stimulated in vitro by the recombinant HIVtop4 protein. Data show IFN-γ cytokine levels in independent experiments (mean ± SD) (3a). IL4 (Th2) secretion from splenocytes of administrated mice recalled with polytopes was measured and conjugate adjuvanted with alum indicated higher frequency of IL4-secreting (3b).
Figure 4. Lymphoproliferative response. Spleen cells were re-stimulated in vitro with 10 μg/ml of polytope in the presence of BrdU. Results (mean ± SD) are shown as S.I.
Figure 5. Specific lysis of the polytope-pulsed target cells (p815) was measured by the LDH assay. In accordance with the results of IgG subclass and IFN-γ, conjugated formulation indicated a relatively higher Th1-oriented immune response.
