Figures & data
Table 1. Primer sequences used for real-time PCR (5′–3′).
Figure 2. Scanning electron micrograph of polycaprolactone nano-fibre (original magnification A × 100, B × 1000). CD34+ cord blood cells culture in 2D medium (original magnification C × 400) were growing in PCL nano-fibre coated with fibronectin after 10 days (original magnification D × 1000).
![Figure 2. Scanning electron micrograph of polycaprolactone nano-fibre (original magnification A × 100, B × 1000). CD34+ cord blood cells culture in 2D medium (original magnification C × 400) were growing in PCL nano-fibre coated with fibronectin after 10 days (original magnification D × 1000).](/cms/asset/6f9dc5b6-6b3d-4426-a2df-ce58b2540261/ianb_a_1337026_f0002_c.jpg)
Table 2. Expression of surface markers on cells before and after expansion under different conditions.
Figure 5. Haematopoietic stem cell (CD34+), granulocytes (CD13 and CD33), lymphocytes (CD2, CD3 and CD19) and monocyte (CD14) markers in unexpanded cells immediately after extraction, 2D and 3D cell culture systems after 10 days expansion.
![Figure 5. Haematopoietic stem cell (CD34+), granulocytes (CD13 and CD33), lymphocytes (CD2, CD3 and CD19) and monocyte (CD14) markers in unexpanded cells immediately after extraction, 2D and 3D cell culture systems after 10 days expansion.](/cms/asset/e3fdbf6c-b107-4d93-ae82-f72b21d5404e/ianb_a_1337026_f0005_b.jpg)
Figure 6. Image of BFU-E/CFU-E (A), CFU-GM (B) and CFU-GEMM (C) based on total CFU analysed for cells expanded in different culture system for 10 days.
![Figure 6. Image of BFU-E/CFU-E (A), CFU-GM (B) and CFU-GEMM (C) based on total CFU analysed for cells expanded in different culture system for 10 days.](/cms/asset/20698821-2391-4032-b11b-71eaceba1273/ianb_a_1337026_f0006_c.jpg)
Figure 7. Number of different colony in unexpanded CD34+ cells (primary), 2D and 3D cell culture systems after 10 days culture.
![Figure 7. Number of different colony in unexpanded CD34+ cells (primary), 2D and 3D cell culture systems after 10 days culture.](/cms/asset/f3ade32b-0597-44f4-a645-5772ce974217/ianb_a_1337026_f0007_b.jpg)
Table 3. Number of colony before and after expansion under different conditions.