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Original Articles

Rapid biosynthesis of Bauhinia variegata flower extract-mediated silver nanoparticles: an effective antioxidant scavenger and α-amylase inhibitor

, , , , , , , & show all
Pages 1488-1494 | Received 09 Jun 2017, Accepted 28 Aug 2017, Published online: 08 Sep 2017

Figures & data

Figure 1. Illustration of the green synthesis of AgNPs using B. variegata flower extract. Descriptive graph showing (a) Bauhinia variegata flower; (b) formation of silver nanoparticles: (i) silver nitrate solution; (ii) B. variegata flower extract (BVFE) without silver nitrate; (iii) BVFE with silver nitrate solution; (c) UV–vis absorption spectrum.

Figure 1. Illustration of the green synthesis of AgNPs using B. variegata flower extract. Descriptive graph showing (a) Bauhinia variegata flower; (b) formation of silver nanoparticles: (i) silver nitrate solution; (ii) B. variegata flower extract (BVFE) without silver nitrate; (iii) BVFE with silver nitrate solution; (c) UV–vis absorption spectrum.

Figure 2. Structural characterization of BVF-AgNPs. (a) TEM image; (b) energy-dispersive X-ray spectrum; (c) XRD pattern showing crystallinity structure of AgNPs; (d) zeta potential stability measurement obtained for biosynthesized B. variegata flower extract-mediated AgNPs (BVF-AgNPs).

Figure 2. Structural characterization of BVF-AgNPs. (a) TEM image; (b) energy-dispersive X-ray spectrum; (c) XRD pattern showing crystallinity structure of AgNPs; (d) zeta potential stability measurement obtained for biosynthesized B. variegata flower extract-mediated AgNPs (BVF-AgNPs).

Figure 3. FTIR spectrum of B. variegata flower extract-mediated silver nanoparticles (BVF-AgNPs).

Figure 3. FTIR spectrum of B. variegata flower extract-mediated silver nanoparticles (BVF-AgNPs).

Figure 4. Antioxidant activities of BVFE and BVF-AgNPs. (a) DPPH radical scavenging activity and (b) FRAP activity in the presence of various concentration of B. variegata flower extract (BVFE) and B. variegata flower extract-mediated silver nanoparticles (BVF-AgNPs) synthesized are shown. BVF-AgNPs showed powerful antioxidant activity than BVFE. Results are expressed as mean ± SD (n = 6).

Figure 4. Antioxidant activities of BVFE and BVF-AgNPs. (a) DPPH radical scavenging activity and (b) FRAP activity in the presence of various concentration of B. variegata flower extract (BVFE) and B. variegata flower extract-mediated silver nanoparticles (BVF-AgNPs) synthesized are shown. BVF-AgNPs showed powerful antioxidant activity than BVFE. Results are expressed as mean ± SD (n = 6).

Figure 5. Effect of BVF-AgNPs on α-amylase enzyme activity. (a) Percentage inhibition of α-amylase activity by acarbose, B. variegata flower extract (BVFE) and B. variegata flower extract-mediated silver nanoparticles (BVF-AgNPs) synthesized at various concentrations are shown; (b) LB plot to understand the mode of inhibition by BVF-AgNPs; (c) Dixon’s plot for determining the inhibitor constant of BVF-AgNPs.

Figure 5. Effect of BVF-AgNPs on α-amylase enzyme activity. (a) Percentage inhibition of α-amylase activity by acarbose, B. variegata flower extract (BVFE) and B. variegata flower extract-mediated silver nanoparticles (BVF-AgNPs) synthesized at various concentrations are shown; (b) LB plot to understand the mode of inhibition by BVF-AgNPs; (c) Dixon’s plot for determining the inhibitor constant of BVF-AgNPs.

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