In vitro neurotoxicity by ropivacaine is reduced by silencing Cav3.3 T-type calcium subunits in neonatal rat sensory neurons

Figures & data

Table 1. β-Actin, CaMKIIγ, Cav3.3 mRNA primer sequence.

Gene	Primer sequence	Product size (bp)
β-actin	F: 5′-CACGATGGAGGGGCCGGACTCATC-3′ R: 5′-TAAAGACCTCTATGCCAACACAGT-3′	240
Cav3.3	F: 5′-GACCAGCAGCCAGTGACGAA-3′ R: 5′-CACGACCACGCCCACAAACA-3′	108
CaMKIIγ	F: 5′-TTGCTGCTGGCGAGTAAATG-3′ R: 5′-TAGGGATCTTTCCTCAAGACCTCA-3′	149

Figure 1. The cell viability of the cell [

%, n=8]. Cell viability in every group was normalized to the normal group. ^ap < .05 vs normal group, ^bp < .05 vs normal + R group.

Figure 2. The cell death rate detected with Hoechst–PI method [

%, n=8]. (A) The representative figure of the death rate in every group with Hoechst-PI. B: The results of the death rate in every group, ^ap < .05 vs normal group, ^bp < .05 vs normal + R group.

Figure 3. Cell apoptosis rate [

%, n=8]. (A) The represent figure of the cell apoptosis rate detected with flow cytometry. (B) The results of the apoptosis rate in every group, ^ap < .05 vs normal group, ^bp < .05 vs normal + R group.

Figure 4. The effects of ropivacaine hydrochloride on Cav3.3 mRNA expression [

%, n=6]. ^ap < .05 vs normal group, ^bp < .05 vs normal + R group.

Figure 5. The effects of ropivacaine hydrochloride on CaMKIIγmRNA expression [

% n=6]. ^ap < .05 vs normal group, ^bp < .05 vs normal + R group.

Figure 6. The effects of ropivacaine hydrochloride on Cav3.3 and CaMKIIγ protein expression detected with western blotting [

% n=6]. (A) The present band detected with western blotting. Lane 1,7: normal group; Lane 2,8: empty vector group; Lane 3: pAd-Cav3.3 group; Lane 4,10: normal + R group; Lane 5,11: empty vector + R group; Lane 6: pAd-Cav3.3 + R group; Lane 9: pAd-shRNA group; Lane 12: pAd-shRNA + R group. (B) The statistical result of Cav3.3 protein. (C) The statistical result of CaMKIIγ protein. ^ap < .05 vs normal group, ^bp < .05 vs normal + R group.