Pharmacokinetics, biodistribution and receptor mediated endocytosis of a natural Angelica sinensis polysaccharide

Figures & data

Figure 1. Analysis of FA and ASP using HPGPC method.

Figure 2. Representative chromatograms for the determination of FA in plasma (A) and various tissues (B: liver; C: stomach; D: spleen; E: heart; F: intestines) by HPGPC. (1) Blank plasma or tissue; (2) Blank plasma or tissue spiked with standard FA solution; (3) Plasma or tissue samples collected after intravenous injection of FA; (4) FA dissolved in phosphate buffer (pH =7.4).

Table 1. Calibration curves for FA in rat plasma and tissues (n = 5).

Tissues	Standard curves	Correlation coefficients	Linear ranges (μg/mL)
Plasma	Y = 998.91C–136.08	0.9998	0.25–40.00
Heart	Y = 418.34C + 11.45	0.9996	0.25–20.00
Liver	Y = 1116.58C–179.67	0.9992	0.25–40.00
Spleen	Y = 561.37C + 18.98	0.9992	0.25–20.00
Lung	Y = 572.38C + 19.17	0.9997	0.25–20.00
Kidney	Y = 524.80C + 16.19	0.9995	0.25–20.00
Brain	Y = 536.50C + 17.36	0.9996	0.25–20.00
Stomach	Y = 411.07C–10.31	0.9994	0.25–20.00
Intestines	Y = 446.06C–17.24	0.9998	0.25–20.00

Table 2. Distribution of FA in tissues (n = 5)..

	Concentrations (μg/mL, mean ± SD)
Tissues	30	60	120
Heart	13.32 ± 3.04	8.00 ± 1.23	4.65 ± 1.67
Liver	111.66 ± 20.91	287.03 ± 77.71	176.98 ± 53.57
Spleen	12.33 ± 3.78	4.96 ± 1.77	4.64 ± 0.64
Lung	24.54 ± 7.43	9.63 ± 1.64	4.28 ± 1.44
Kidney	46.91 ± 7.87	10.99 ± 3.38	2.37 ± 0.17
Brain	1.88 ± 0.49	1.63 ± 0.36	nd
Stomach	16.57 ± 3.89	3.54 ± 1.20	3.40 ± 0.63
Intestines	18.03 ± 3.90	6.84 ± 1.85	6.84 ± 3.58

nd: not detected.

Figure 3. ASP concentration versus time profile following intravenous administration. Data are presented as mean ± SD (n = 6).

Figure 4. Hepatocytes uptake of FA. (A) Distributions of FA and FD in liver parenchymal and non-parenchymal cells 2 h after intravenous injection at a dose of 6 mg/kg. Values are given as mean ± SD (n = 3). (B) Fluorescence microscopic examination of paraffin sections of mice liver 2 h after intravenous injection of FA (B1) and FP (B2). The bar represents a length of 50 μm. (C) Fluorescence intensity of FA in liver parenchymal cells. (D) The uptake rate of FA in liver parenchymal cells. Values are given as mean ± SD (n = 3).

Figure 5. (A) Uptake rate of cells treated with different concentrations of FA (n = 3). ▪, HepG2 cells, •, Bel-7402 cells, ▲, Hela cells; (B) Effect of NGA on the uptake of FA to cells (n = 3); (C) Effect of NGA or dextran on the uptake of FA to mice liver (n = 3).

Figure 6. Preparation of ^99mTc-DTPA-ASP. (A) The reaction route. (B) IR spectra of ASP and DTPA-ASP. (C) ¹H-NMR spectra of ASP, DTPA and ASP-DTPA. (D) Chromatographic separation of ^99mTc-DTPA-ASP and Na^99mTcO₄ using physiological as mobile phase.

Figure 7. Planar images of rabbit after ear intravenous injection of ^99mTc-DTPA-ASP with or without NGA. The upper row is control group; low row is blocking group.