Controlled release of mitomycin C from PHEMAH–Cu(II) cryogel membranes

Figures & data

Table 1. Parameters for cryogel membrane synthesis.

Notation forcryogels	(µg) MMC/g polymer	nHEMA/nMBAAm	Polymerizationtemperature (°C)
MMC-ICM1	50	1:16	−14
MMC-ICM2	50	1:10	−14
MMC-ICM3	50	1:8	−14
MMC-ICM4	50	1:4	−14
MMC-ICM5	50	1:4	−18
MMC-ICM6	50	1:4	−20
MMC-ICM7	50	1:4	−24
NICM	0	1:4	−14
PHEMACM	0	1:4	−14

Figure 1. (A) The structure of MAH monomer. (B) The UV-spectrum of MAH–Cu(II)–MMC complex. (C) Hypothetical model for the MAH–Cu(II)–MMC complex.

Figure 2. FTIR-ATR spectra of MMC-ICM and PHEMACM.

Figure 3. The SEM images of MMC-ICMs synthesized at (A) − 14 °C, (B) − 18 °C, (C) − 20 °C and (D) − 22 °C.

Figure 4. The SEM photograph of MMC-ICMs synthesized with different monomer ratios at −14 °C: (A) 1:16, (B) 1:10, (C) 1:8 and (D) 1:4 n_HEMA/n_MBAAm.

Table 2. Comparison of the properties of cryogel membranes.

Notation for cryogels	nHEMA/nMBAAm	Polymerization temperature (°C)	Swelling ratio %	Surface area m^2/g	Pore diameter (µm)
MMC-ICM1	1:16	−14	757.9 ± 0.1	30.8	20–50
MMC-ICM2	1:10	−14	761.2 ± 0.1	33.3
MMC-ICM3	1:8	−14	803.5 ± 0.1	35.5
MMC-ICM4	1:4	−14	898.4 ± 0.1	45.2
MMC-ICM5	1:4	−18	831.3 ± 0.1	38.1
MMC-ICM6	1:4	−20	789.1 ± 0.1	33.6
MMC-ICM7	1:4	−24	769.1 ± 0.1	30.2
N-ICM	1:4	−14	705.2 ± 0.1	21.6

Figure 5. Effect of cross-linker ratio on the MMC release (BPS buffer, 37 °C, n = 3).

Figure 6. Effect of loaded drug concentration on the MMC release from MMC-ICM4.

Figure 7. Effect of polymerization temperature on MMC delivery from the cryogel membranes.

Table 3. Release kinetics data for MMC-ICMs.

Notation for cryogels	n	k	R^2
MMC-ICM1	0.46	0.71	0.973
MMC-ICM2	0.44	0.7	0.980
MMC-ICM3	0.71	0.54	0.875
MMC-ICM4	0.81	0.32	0.989
MMC-ICM5	0.57	0.06	0.95
MMC-ICM6	0.6	0.12	0.96
MMC-ICM7	0.56	0.33	0.901

Figure 8. The viability of cell lines treated with MMC-ICMs and PHEMA-ICM (50, 75 and 100 μg MMC/mL) after 12 and 24 h.