Figures & data
Table 1. Specifications of primer sequences used for quantitative real-time PCR.
Figure 1. Morphological changes in 2 D culture. (a) hiPSCs in DMEM (control group) and (b) hiPSCs in IPC differentiation media. Scale bars are 100 µm.
Figure 2. Morphological changes in 3 D culture. (a) Unseeded scaffold. Interconnected pores are observed. (b) hiPSCs-seeded scaffold in DMEM (control group). The cells show spindle-shape morphology. (c) hiPSCs-seeded scaffold in IPC differentiation media. The cells form spherical clusters of round cells. Scale bars are 10 and 30 µm, respectively.
Figure 3. MTT cell proliferation assay of hiPSCs on PLLA/PVA scaffold (3 D) and tissue culture polystyrene (2 D) during 1, 3, 5 and 7 days of culture. *p < .05.
Figure 4. Relative gene expression in end stage derived IPCs. The expression levels of pancreatic transcription factors such as Pdx1, Ngn3, insulin, glucagon and Glut2 were analyzed in each group of differentiation into IPCs. Gene transcripts of 3 D group are compared with the 2 D group. Relative levels of gene expression were normalized to the human β2M. The values in each graph are represented as mean ± SD. *p < .05, **p < .01 and ****p < .0001.
Figure 5. Immunocytochemical analysis in end stage derived IPCs. Immunofluorescence analysis detected nuclei localization of PDX1 and cytoplasmic localization of insulin in differentiated IPCs at day 14 in the 3D group (a) and 2 D group (b). Counter-staining of nucleus was performed by DAPI. Images were obtained by a fluorescence microscope. Scale bars are 100 µm.
Figure 6. In vitro insulin and C-peptide response assay in end stage derived IPCs. (a and b) insulin secretion and C-peptide release changes in two groups of IPCs and control group in response to various concentrations of glucose from 5.5 to 25 mM. (c) Intracellular insulin content in each concentration of glucose that was normalized with total cellular protein. Values are expressed as mean ± SD (n = 6). *p < .05, **p < .01 and ***p < .001.
