1,883
Views
28
CrossRef citations to date
0
Altmetric
Research Article

The immunomodulatory effects of Zn-incorporated micro/nanostructured coating in inducing osteogenesis

, , , , , , , & show all
Pages 1123-1130 | Received 24 Nov 2017, Accepted 24 Feb 2018, Published online: 08 Mar 2018

Figures & data

Figure 1. SEM morphologies of MAO (a, b) and MHTZn coating (c, d).

Figure 1. SEM morphologies of MAO (a, b) and MHTZn coating (c, d).

Figure 2. Morphologies of attached RAW264.7 cells cultured on MAO coating for one day (a), and three days (b), and on MHTZn coating for one day (c) and three days (d).

Figure 2. Morphologies of attached RAW264.7 cells cultured on MAO coating for one day (a), and three days (b), and on MHTZn coating for one day (c) and three days (d).

Figure 3. Skeleton and nuclei staining for MAO group at day 1 (a), day 3 (b), and day 5 (c); MHTZn group at day 1 (d), day 3 (e), and day 5 (f). Cell viability of RAW264.7 cells on MAO and MHTZn coatings tested by CCK-8 (g). Values are mean ± SD (n=3); * depicts statistical differences; * p < .05 vs. MAO.

Figure 3. Skeleton and nuclei staining for MAO group at day 1 (a), day 3 (b), and day 5 (c); MHTZn group at day 1 (d), day 3 (e), and day 5 (f). Cell viability of RAW264.7 cells on MAO and MHTZn coatings tested by CCK-8 (g). Values are mean ± SD (n=3); * depicts statistical differences; * p < .05 vs. MAO.

Figure 4. Inflammatory secretions of RAW264.7 cells cultured on MAO and MHTZn coatings for two days. Values are mean ± SD (n = 3); *statistical differences; *p < .05, **p < .01 vs. MAO.

Figure 4. Inflammatory secretions of RAW264.7 cells cultured on MAO and MHTZn coatings for two days. Values are mean ± SD (n = 3); *statistical differences; *p < .05, **p < .01 vs. MAO.

Figure 5. SaOS-2 cells after culturing in the MAO conditioned media for one day (a) and three days (c), in the MHTZn conditioned media for one day (b) and three days (d), showing the nuclei stained by DAPI and the cytoskeleton stained by phalloidin with TRITC.

Figure 5. SaOS-2 cells after culturing in the MAO conditioned media for one day (a) and three days (c), in the MHTZn conditioned media for one day (b) and three days (d), showing the nuclei stained by DAPI and the cytoskeleton stained by phalloidin with TRITC.

Figure 6. Cell viability of SaOS-2 cells cultured in the MAO and MHTZn conditioned media tested by CCK-8. Values are mean ± SD (n = 3).

Figure 6. Cell viability of SaOS-2 cells cultured in the MAO and MHTZn conditioned media tested by CCK-8. Values are mean ± SD (n = 3).

Figure 7. ALP activity of SaOS-2 cells cultured in MAO and MHTZn conditioned media for 7 days. MAO group used as the control (a), MHTZn group (b) and OD values (c). Values are mean ± SD (n=3);   depicts statistical differences; * p < .05 vs. MAO.

Figure 7. ALP activity of SaOS-2 cells cultured in MAO and MHTZn conditioned media for 7 days. MAO group used as the control (a), MHTZn group (b) and OD values (c). Values are mean ± SD (n=3);   depicts statistical differences; * p < .05 vs. MAO.

Figure 8. Extracellular mineralization of SaOS-2 cells cultured in the MAO and MHTZn conditioned media for 14 days. MAO group used as the control (a), MHTZn group (b) and OD values (c). Values are mean ± SD (n = 3); * depicts statistical differences; * p < 0.05 vs. MAO.

Figure 8. Extracellular mineralization of SaOS-2 cells cultured in the MAO and MHTZn conditioned media for 14 days. MAO group used as the control (a), MHTZn group (b) and OD values (c). Values are mean ± SD (n = 3); * depicts statistical differences; * p < 0.05 vs. MAO.
Supplemental material

R._ZHANG_ET_AL_supplementary_material.zip

Download Zip (60.9 KB)

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.