CD133-targeted delivery of self-assembled PEGylated carboxymethylcellulose-SN38 nanoparticles to colorectal cancer

Figures & data

Figure 1. Chemical structure of PEG-AcCMC-SN38.

Figure 2. (A) ¹HNMR spectra of Na-CMC in deionized water, (B) ¹HNMR spectrum of Ac-CMC in DMSO-d6, and (C) ¹HNMR spectrum of PEG-AcCMC-SN38 in chloroform.

Figure 3. (A) Critical micelle concentration of PEG-AcCMC-SN38. (B) Field emission-scanning electron microscopy (FE-SEM) image of PEG-AcCMC-SN38 self-assembled nanoparticles. (C) Release profile of SN38 from PEG-AcCMC-SN38 self-assembled nanoparticles in PBS (pH: 7.4) containing 30% of FBS and citrate buffer (pH: 5.5) for 96 h.

Table 1. Characteristics of targeted and nontargeted self-assembled structures in terms of size, polydispersity index (PDI), and zeta potential.

Sample name	Size (nm)	PDI	Zeta potential (mv)
PEG-AcCMC-SN38	169.9 ± 3.5	0.11	–21.6
Apt-PEG-AcCMC-SN38	171.5 ± 2.6	0.13	–12.1

Figure 4. Flow cytometry histogram showing the cellular uptake of Rhodamine 6G encapsulated PEG-AcCMC-SN38, Apt-PEG-AcCMC-SN38 and Apt-PEG-AcCMC-SN38 + free aptamer and untreated cells as control in CD133 positive cells, HT-29 cells (A) CD133 negative cell, (B) CHO cells.

Figure 5. Fluorescent microscopy images showing the cellular uptake of Rhodamine 6G encapsulated Apt-PEG-AcCMC-SN38, PEG-AcCMC-SN38 and Apt-PEG-AcCMC-SN38 + free aptamer as a competitive ligand in (A) CD133 positive cells, HT-29 cells (B) CD133 negative cells, CHO cells.

Figure 6. Cytotoxicity of Apt-PEG-AcCMC-SN38, PEG-AcCMC-SN38 and the free drug in (A) HT-29 cells as CD133 positive cell and (B) CHO cells as CD133 negative cell.

Figure 7. Fluorescent microscopy images showing the cytotoxic effect of different groups compared to control in HT-29 cells as CD133 positive cells and in CHO cells as CD133 as negative cells.