A perfect stimuli-responsive magnetic nanocomposite for intracellular delivery of doxorubicin

Figures & data

Scheme 1. Synthesis of poly(HEMA) via RAFT polymerization technique.

Scheme 2. Synthesis of poly(HEMA-b-NIPAM).

Scheme 3. Synthesis of poly(HEMA-b-NIPAM-b-DMAEMA).

Scheme 4. Synthesis of poly(SEMA-b-NIPAM-b-DMAEMA).

Figure 1. FT-IR spectra of poly(HEMA), poly(HEMA-b-NIPAM), poly(HEMA-b-NIPAM-b-DMAEMA) and poly(SEMA-b-NIPAM-b-DMAEMA) (a); nanocomposite and Fe₃O₄ (b).

Figure 2. ¹HNMR spectra of poly(HEMA) (a); poly(HEMA-b-NIPAM) copolymer (b); poly(HEMA-b-NIPAM-b-DMAEMA) copolymer (c).

Figure 3. GPC traces of poly(HEMA), poly(HEMA-b-NIPAM) and poly(HEMA-b-NIPAM-b-DMAEMA) triblock copolymers.

Table 1. Data analyses of poly(HEMA), poly(HEMA-b-NIPAM) and poly(HEMA-b-NIPAM-b-DMAEMA) by GPC and ¹HNMR.

Sample	Mn^a	Mn^b	Mw^a	PDI^a
	(g/mol)	(g/mol)	(g/mol)
	(GPC)	(^1HNMR)	(GPC)	(GPC)
Poly(HEMA)	10300	10429	11701	1.14
Poly(HEMA-b-NIPAM)	17802	17086	21200	1.19
Poly(HEMA-b-NIPAM-b-DMAEMA)	26875	22944	33862	1.26

Figure 4. FESEM image of the triblock copolymer (a), Fe₃O₄ (b) and poly(SEMA-b-NIPAM-b-DMAEMA)/Fe₃O₄ magnetic composite (c).

Figure 5. X-ray diffraction patterns (a), magnetization curve (b) and thermogravimetry (TG) (c) of poly(SEMA-b-NIPAM-b-DMAEMA)/Fe₃O₄ magnetic nanocomposite and Fe₃O₄ nanoparticles. The blue spectra stood for Fe₃O₄ and red spectra was for poly(SEMA-b-NIPAM-b-DMAEMA)/Fe₃O₄ magnetic nanocomposite.

Figure 6. Particle size dependent on pH (a) and temperature (b) for poly(SEMA-b-NIPAM-b-DMAEMA)/Fe₃O₄ magnetic nanocomposite.

Figure 7. The photographs (a) and cumulative in vitro release profiles (b) of DOX@poly(SEMA-b-NIPAM-b-DMAEMA)/Fe₃O₄ magnetic nanocomposite.

Figure 8. The cell viability study of magnetic nanocomposite (a), DOX@magnetic nanocomposite, and free DOX with different concentrations in time period of 24 (a), 48 (b) and 72 (c) h against breast cancer (MCF7) cell line.

Figure 9. DAPI staining of untreated human breast epithelial adenocarcinoma (MCF-7) cells (control), treated with DOX and DOX@magnetic nanocomposite (c).

Figure 10. Cellular uptake images of human breast epithelial adenocarcinoma (MCF7) cells after incubation with rhodamine-labelled free DOX and DOX@magnetic nanocompositeafter 0.5 and 3 h.