Kinetic studies on oxygen releasing of HBOC and red blood cells as fluids and factors affecting the process

Figures & data

Figure 1. Schematic diagram of the oxygen-releasing apparatus.

Table 1. General pO₂ measurement conditions for Hb- or RBC-releasing oxygen.

No.	Items	PO2 measurement conditions for Hb- or RBC-releasing oxygen
1	RBCs	In physiological saline
2	Hb	In PBS buffer
3	Hb	0, 3, 6, 9 and 12 mmol/L of phytic acid (PHY) solution, respectively
4	RBCs	Mass fraction 2, 4, 6 and 8% of sodium chloride solution, respectively
5	RBCs	200, 500, 800, 1100 mPa•s of viscosity levels modified with sodium alginate, in physiological saline
6	Hb	200, 500, 800, 1100 mPa•s of viscosity levels modified with sodium alginate in pBS buffer

PO₂ measurements of RBC- or Hb-releasing oxygen were carried out at a Hb concentration of 5 g/dL at 37 °C, pH 7.4.

Figure 2. The oxygen-releasing curves of Hb and RBCs (pO₂ of Hb- or RBC-releasing oxygen was determined at a Hb concentration of 5 g/dL, at 37 °C, pH 7.4, (i) for RBCs in physiological saline; (ii) for Hb in PBS).

Figure 3. The oxygen-releasing rate curves of Hb and RBCs (PO₂ of Hb- or RBC-releasing oxygen was determined at a Hb concentration of 5 g/dL, at 37 °C, pH 7.4, (i) for RBCs in physiological saline; (ii) for Hb in PBS. Dt/dpO₂ in the vertical axis referred to the oxygen-releasing rate obtained by taking derivatives of time to pO₂).

Figure 4. The pO₂–time oxygen-releasing curves of Hb in different concentrations (PO₂ of Hb releasing oxygen was measured in 0, 3, 6, 9 and 12 mmol/L of PHY solutions, at a Hb concentration of 5 g/dL at 37 °C, pH 7.4).

Figure 5. The oxygen-releasing rate curves of Hb in different concentrations of PHY solution (PO₂ of Hb-releasing oxygen was measured in 0, 3, 6, 9 and 12 mmol/L of PHY solutions, respectively, at a Hb concentration of 5 g/dL at 37 °C, pH 7.4. Dt/dpO₂ in the vertical axis referred to the oxygen-releasing rate obtained by taking derivatives of time to pO₂).

Figure 6. The pO₂–time oxygen-releasing curves of RBCs under different osmotic pressures (PO₂ of RBC-releasing oxygen was measured in mass fraction 2, 4, 6 and 8% of sodium chloride solution, respectively, at a Hb concentration of 5 g/dL at 37 °C, pH 7.4).

Figure 7. The oxygen-releasing rate curves of RBCs under different osmotic pressures (PO₂ of RBC-releasing oxygen was measured in mass fraction 2, 4, 6 and 8% of sodium chloride solution, respectively, at a Hb concentration of 5 g/dL at 37 °C, pH 7.4. Dt/dpO₂ in the vertical axis referred to the oxygen-releasing rate obtained by taking derivatives of time to pO₂).

Figure 8. The pO₂–time oxygen-releasing curves of Hb with different viscosities (PO₂ of Hb-releasing oxygen was measured at different viscosity levels of 200, 500, 800, 1100 mPa•s, respectively, at a Hb concentration of 5 g/dL at 37 °C, pH 7.4).

Figure 9. The oxygen-releasing rate curves of Hb with different viscosities (PO₂ of Hb-releasing oxygen was measured at different viscosity levels of 200, 500, 800, 1100 mPa•s, respectively, at a Hb concentration of 5 g/dL at 37 °C, pH 7.4. Dt/dpO₂ in the vertical axis referred to the oxygen-releasing rate obtained by taking derivatives of time to pO₂).

Figure 10. The pO₂–time oxygen-releasing curves of RBCs with different viscosities (PO₂ of RBC-releasing oxygen was measured at different viscosity levels of 200, 500, 800, 1100 mPa•s, respectively, at a Hb concentration of 5 g/dL at 37 °C, pH 7.4).

Figure 11. The oxygen-releasing rate curves of RBCs with different viscosities (PO₂ of RBC-releasing oxygen was measured at different viscosity levels of 200, 500, 800, 1100 mPa•s, respectively, at a Hb concentration of 5 g/dL at 37 °C, pH 7.4. Dt/dpO₂ in the vertical axis referred to the oxygen-releasing rate obtained by taking derivatives of time to pO₂).