PEG-interpenetrated genipin-crosslinked dual-sensitive hydrogel/nanostructured lipid carrier compound formulation for topical drug administration

Figures & data

Table 1. Compositions of the formation of PEG-penetrated F127/CMCS hydrogel.

Sample	CMCS (%,w/v)	F127 (%,w/v)	GP (mg)	PEG4000 (%,w/v)	Water (ml)
1	3.0	1.0	2	0	10
2	3.0	1.0	2	0.5	10
3	3.0	1.0	2	1.0	10
4	3.0	1.0	2	1.5	10
5	3.0	1.0	2	2.0	10

Figure 1. TEM image of ALA-NLC.

Figure 2. TGA thermogram of ALA and soybean lecithin.

Table 2. Physicochemical parameters of formulated ALA-NLC.

Formulation	Z-ave (nm)	PDI	ZP (mV)	EE (%)
ALA-NLC	60.1 ± 1.54	0.279 ± 0.03	−23.77 ± 0.56	88.62 ± 1.74

Figure 3. FTIR spectrum of (A) P407, (B) CMCS, (C) GP-CMCS/P407 hydrogel and (D) GP.

Figure 4. SEM images of the lyophilised PEG-interpenetrated CMCS/P407 hydrogels. (A) 0% PEG; (B) 0.5% PEG; (C) 1.5%PEG; (D) 2.0% PEG.

Figure 5. Rheological characterisation of five PEG-interpenetrated CMCS/P407 hydrogel. (A) Shear stress; (B) Viscosity variation.

Figure 6. Swelling characteristic of the hydrogels CMCS/P407 hydrogel (a) in different test conditions (b) with gradient amount of PEG 4000 at pH 6.5, 35 °C (mean ± SD, n = 3).

Figure 7. The in-vitro dissolution profiles of α-lipoic acid from various formulations at pH 6.5, 35 °C (mean ± SD, n = 3).

Figure 8. After 24 h incubation, CCK8 cell viability assay results of the hydrogels (mean ± SD, n = 3).