Argyreia nervosa (Samudra pala) leaf extract mediated silver nanoparticles and evaluation of their antioxidant, antibacterial activity, in vitro anticancer and apoptotic studies in KB oral cancer cell lines

Figures & data

Figure 1. (A) Argyreia nervosa plant. (B): (a) Plant extract of A. nervosa; (b) Green synthesised An-AgNPs by Plant extract of A. nervosa. (C) UV-VIS absorption spectra of An-AgNPs synthesised from A. nervosa plant extract with 0.02 M silver nitrate.

Figure 2. (A) FTIR spectrum of the A. nervosa leaf extract. (B) FTIR spectrum of the green synthesised An-AgNPs.

Figure 3. (A) Particle size distribution curve of green synthesised An-AgNPs. (B) Zeta potential of green synthesised An-AgNPs.

Figure 4. (A) TEM images of green synthesised An-AgNPs at different magnification (a) 200 Kx (b)100 Kx (c) 50Kx (d) 50Kx (e)20 Kx and (f) SAED pattern showed four diffraction rings. (B) EDX analysis of green synthesised An-AgNPs.

Table 1. Antimicrobial activity of An-AgNPs.

		Zone of Inhibition (ZOI) mm
S. No.	Name of species	10 mcg	20 mcg	30 mcg	40 mcg	Amoxycilin (30 mcg)
1	E. coli (D)	15	17	20	24	20
2	E. coli (R)	28	30	32	36	33
3	E. coli (M)	16	19	21	25	31
4	E. coli	9	14	16	20	24
5	S. aureus	15	24	25	27	19
6	B. megatorium	30	32	34	36	39

Table 2. DPPH antioxidant activity An-AgNPs.

Concentration (µl)	A nervosa leaf extract	An- AgNPs	Ascorbic acid
50	23.12	37.16	42.67 ± 0.26
100	36.64	48.18	52.23 ± 0.07
150	48.6	56.34	58.64 ± 0.56
200	50.28	62.22	64.34 ± 0.67
250	54.12	69.96	70.26 ± 0.67

Figure 6. (A) MTT assay of cytotoxic studies of green synthesised An-AgNPs on KB-human oral carcinoma cell line and its morphological changes of KB cells observed under an inverted light microscope. (a) Untreated KB cell line, (b) KB cell line cells treated with 25 µg of An-AgNPs, (c) KB-cell line treated with 50 µg of An-AgNPs, (d) KB cell lines treated with 100 µg of An-AgNPs, (e) KB cell lines treated with 200 µg of An-AgNPs, and (f) Standard control(Camptothecin 7.5 uM). (B and C) Comparative percentage of Cell viability studies of green synthesised An-AgNPs on KB-human oral carcinoma cell line by MTT assay and the IC₅₀ value is calculated to be = 58.64 µl/mL.

Figure 7. (A,B,C) Cycle cell assay of green synthesised An AgNPs on KB-human oral carcinoma cell line.

Data availability statement

All the data related to our research article is present in the manuscript submitted