Naturally infected Aedes aegypti collected during a Zika virus outbreak have viral titres consistent with transmission

Figures & data

Table 1. Results of real-time-quantitative Polymerase chain reaction assays of mosquito pools collected during a Mexican Zika virus outbreak.

Pool ID	Mosquito species	Gender	#/Pool	CT Value	Extrapolated infectious titre from standard curve (FFU/pool)^a	GenBank Acc. No.
3-7	A. aegypti	Female	10	14.12	1.1×10^6^a	N/A
1-65		Female	6	14.82	6.6×10^5^a	N/A
1-7		Female	6	15.14	5.3×10^5^a	KX247632.1
1-71		Female	17	15.472	4.2×10^5^a	N/A
1-21		Female	10	16.09	2.7×10^5^a	N/A
1-44		Female	1	16.87	1.6×10^5^a	KX856011.1
1-19		Female	10	17.84	8.1×10^4	N/A
1-26		Female	5	18.43	5.4×10^4	N/A
2-81		Female	1	19.27	3.0×10^4	KX446590.2
1-69		Female	1	19.52	2.5×10^4	N/A
1-10		Female	9	19.81	2.1×10^4	N/A
3-8		Female	10	19.99	1.8×10^4	N/A
1-58		Female	3	20.32	1.4×10^4	N/A
1-20		Female	10	22.03	4.4×10^3	N/A
1-47		Female	1	26.65	1.8×10^2	N/A
2-43	M. titillans	Female	1	33.69	1.3	N/A
2-41	C. quinquefasciatus	Female	1	36.92	0.14	N/A
2-36		Female	1	37.01	0.13	N/A
2-38		Female	1	37.45	0.10	N/A

^aTitres compatible with disseminated, transmission-competent infections, based on previously published laboratory data [10,11].

Karna AK, Azar SR, Plante JA, et al. Colonized Sabethes cyaneus, a Sylvatic New World mosquito species, Shows a low vector competence for Zika virus relative to Aedes aegypti. Viruses. 2018;10; doi:10.3390/v10080434.

 Web of Science ®Google Scholar

Kuo YP, Tsai KN, Luo YC, et al. Establishment of a mouse model for the complete mosquito-mediated transmission cycle of Zika virus. PLoS Negl Trop Dis. 2018;12:e0006417. doi: 10.1371/journal.pntd.0006417

 PubMed Web of Science ®Google Scholar