Lack of cross-neutralization by SARS patient sera towards SARS-CoV-2

Figures & data

Table 1. Summary of serological test results.

Serum group	Sample/Case ID	Years/days post symptom onset	Virus neutralization test^a		ELISA with N protein^b
			SARS-CoV	SARS-CoV-2	SARS-CoV	hSARS-CoV-2
SARS	S1	< 1 year	1:80	<1:20	1.55	1.40
	S2	< 1 year	1:40	<1:20	2.23	2.34
	S3	< 1 year	1:40	<1:20	1.51	1.39
	S4	< 1 year	1:40	<1:20	1.58	1.44
	S5	< 1 year	1:80	<1:20	1.79	1.56
	S6	< 1 year	1:80	<1:20	1.65	1.55
	S7	< 1 year	1:160	<1:20	1.83	2.08
	S8	9 years	1:320	<1:20	0.25	0.27
	S9	9 years	1:320	<1:20	0.37	0.40
	S9	14 years	1:160	<1:20	0.56	0.53
	S10	17 years	1:160	<1:20	0.40	0.47
	S11	17 years	1:80	<1:20	0.95	0.98
	S12	17 years	1:20	<1:20	0.14	0.13
Negative control	N1	NA	<1:20	<1:20	0.06	0.08
	N2	NA	<1:20	<1:20	0.05	0.08
	N3	NA	<1:20	<1:20	0.06	0.09
	N4	NA	<1:20	<1:20	0.06	0.09
COVID-19	C1	6 days	<1:20	<1:20	0.03	0.05
	C1	20 days	<1:20	1:80	0.66	0.71
	C2	4 days	<1:20	<1:20	0.08	0.05
	C2	12 days	<1:20	1:80	2.11	2.28
	C2	18 days	1:20	1:80	2.08	2.42
	C3	4 days	<1:20	1:160	2.02	2.20
	C3	15 days	1:40	1:320	2.24	2.32
	C4	9 days	<1:20	<1:20	0.09	0.09
	C5	11 days	1:20	1:320	2.11	2.34
	C6	7 days	<1:20	<1:20	0.05	0.05
	C7	10 days	1:20	1:160	0.91	0.89

^aAverage Neutralization titers determined from three separate experiments.

^bAverage specific OD readings normalized by dividing the OD readings from human sera by OD readings for each antigen from anti-His monoclonal antibody as both N proteins were expressed with an His-tag.