Dissemination and genome analysis of high-level ceftriaxone-resistant penA 60.001 Neisseria gonorrhoeae strains from the Guangdong Gonococcal antibiotics susceptibility Programme (GD-GASP), 2016–2019

Figures & data

Figure 1. Ceftriaxone decreased susceptibility testing of N. gonorrhoeae in Guangdong, China from 2016 to 2019.

Table 1. Characteristics of penA 60.001 isolates.

(A) Minimum inhibitory concentrations of antibiotics against penA 60.001 isolates.
						MIC	(mg/L)
Number	Strain	City	Year	CRO	CFM	PEN	TET	CIP	SPT	AZM
1	MM08	Maoming	2017	0.25	≥1	8	2	16	16	0.25
2	DG18193	Dongguang	2018	0.5	≥1	2	2	8	16	0.5
3	MM14	Maoming	2018	0.5	≥1	2	4	≥32	16	0.25
4	ZH545	Zhuhai	2018	0.5	≥1	2	4	16	8	0.25
5	DG19112	Dongguang	2019	0.5	≥1	2	2	≥32	8	0.25
6	SS74	Guangzhou	2019	0.5	≥1	≥32	2	16	16	0.06
CRO: Ceftriaxone; CFM: Cefixime; PEN: Penicillin; TET: Tetracycline; CIP: Ciprofloxacin; SPT: Spectinomycin; AZM: Azithromycin
(B) Sequence typing of penA 60.001 strains
Stain name	porB	tbpB	NG-MAST ST
MM14	581	21	1086
MM08	581	21	1086
DG19112	2035	21	20408
ZH545	1132	21	13272
SS74	3723	21	New
DG18193	2682	21	New
Stain name	penA	23srRNA	mtrR	ponA	parC	gyr	porB	NG-STAR ST
MM14	60.001	100	1	1	3	7	8	233
MM08	60.001	100	1	1	3	7	8	233
DG19112	60.001	100	1	1	3	7	8	233
ZH545	60.001	100	1	1	3	7	4	2239
DG18193	60.001	100	1	1	3	7	13	NEW
SS74	60.001	100	1	1	3	21	8	NEW
Stain name	abcZ	adk	aroE	fumC	gdh	pdhC	pgm	MLST ST
SS74	126	39	67	157	148	153	65	1903
MM14	126	39	67	157	148	153	65	1903
DG18193	126	39	67	157	148	153	65	1903
DG19112	126	39	170	157	148	153	65	13943
MM08	126	39	67	157	148	153	65	1903
ZH545	126	39	67	111	148	153	65	7365

Table 2. Resistance genes and virulence factor analysis.

(A) Resistance genes identified among penA 60.001 isolates
	DG18193	DG19112	MM08	MM14	SS74	ZH545
penA	100	100	100	100	100	100
blaTEM-1B	0	0	0	0	100	0
(B) Virulence factors analysis of penA 60.001 isolates
	DG18193	DG19112	MM08	MM14	SS74	ZH545
farB	100	100	100	100	100	100
fbpB	100	100	100	100	100	100
fbpC	100	100	100	100	100	100
hmbR	100	100	100	100	100	100
mntB	100	100	100	100	100	100
mntC	100	100	100	100	100	100
msrA/B(pilB)	100	100	100	100	100	100
mtrC	100	100	100	100	100	100
lbpA	100	100	100	100	100	100
mtrD	100	100	100	100	100	100
mtrE	100	100	100	100	100	100
nspA	100	100	100	100	100	100
pilF	100	100	100	100	100	100
pilG	100	100	100	100	100	100
pilH	100	100	100	100	100	100
pilK	100	100	100	100	100	100
pilM	100	100	100	100	100	100
pilN	100	100	100	100	100	100
pilO	100	100	100	100	100	100
pilP	100	100	100	100	100	100
pilT	100	100	100	100	100	100
pilT2	100	100	100	100	100	100
pilU	100	100	100	100	100	100
pilV	100	100	100	100	100	100
pilW	100	100	100	100	100	100
pilZ	100	100	100	100	100	100
porB	100	100	100	100	100	100
recN	100	100	100	100	100	100
lbpB	100	81.43	100	100	81.43	81.43
katA	99.88	99.88	99.88	99.88	99.88	99.88
pilX	99.8	99.8	99.8	99.8	99.8	99.8
fbpA	99.8	99.8	99.8	99.8	99.8	99.8
hpuA	99.83	99.83	99.83	99.83	99.83	99.83
pilJ	99.68	99.68	99.68	99.68	99.68	99.68
tbpA	99.67	99.67	99.67	99.67	99.67	99.67
pilI	99.51	99.51	99.51	99.51	99.51	99.51
hpuB	98.32	98.32	98.32	98.32	98.32	98.32
pilD	99.3	99.3	99.3	99.3	99.3	99.3
mntA	97.43	0	97.43	97.43	0	0

An absent gene is denoted “0” and a present gene is represented by its “% COVERAGE”.

Figure 2. Maximum likelihood phylogeny of 35 globally disseminated N.gonorrhoeae strains of penA 60.001 clones. The ML phylogeny was constructed based on 254 SNVs in non-recombination regions along the whole genomes. The tree was rooted by mid-rooting method. The six isolates from the current study are coloured red, purple represents known FC428-related clones found in other regions of China, and the rest are foreign penA 60.001 strains. Bootstrap value was labelled as green gradient dot on the branch. The tree scale bar indicates the average number of SNPs per site.